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Transmissible gastroenteritis virus  拉丁名  
VR-1995?  統(tǒng)一編號
Product category：Viruses
Product type類型：Animal virus
Classification： Coronaviridae, Alphacoronavirus, Alphacoronavirus 1
Strain designation：Purdue (attenuated)
Common name：Transmissible gastroenteritis virus
Genome sequenced strain：Yes
Isolation source：Small intestine of infected pig with mild diarrhea
Applications：Agricultural research
Infectious disease research：Enteric disease research
Product format提供形式：Frozen
Storage conditions保存溫度：-70°C or colder
Viral neutralization tests：Characteristics
Comments：Derived by passage of ATCC VR-763 through a 0.2 μM cellulose acetate membrane syringe filter. ATCC VR-763 and ATCC VR-1995 have not been compared for sequence or infectivity.
Handling information：Host
ST (ATCC CRL-1746)
Effects：CPE; cell rounding; cell sloughing
Complete medium：MEM α (ThermoFisher Scientific? catalog 12000063) + 1.68 g/L Sodium Bicarbonate (Millipore Sigma? catalog S5761-500G)
Temperature培養(yǎng)溫度 ：37°C
Atmosphere需氧條件：95% Air, 5% CO2
Incubation：2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
Plate cells 16-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.0001). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes
Derived by passage of ATCC VR-763 through a 0.2 μM cellulose acetate membrane syringe filter. ATCC VR-763 and ATCC VR-1995 have not been compared for sequence or infectivity.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
MEM, Minimum Essential Medium
MOI, Multiplicity of infection
ST cells, Swine testicular cells
Quality control specifications
Volume：1.0 mL
Verification method：Whole-genome Sequencing
History：Depositors
LJ Saif, Ohio Agricultural Research and Development Center
Type of isolate：Animal