ClassificationHerpesviridae, VaricellovirusStrain designationFVRm (modified)Isolation sourceThroat swab from 3 months old cat.Product formatFrozenStorage conditions-70°C or colder
HostCRFK (ATCC CCL-94)
Effectscell rounding; cell sloughing; CPE
Complete medium 培養(yǎng)基 EMEM (ATCC? 30-2003?) + 2% FBS (ATCC? 30-2020?)
Temperature 培養(yǎng)溫度 31°C
Atmosphere 需氧情況 95% Air, 5% CO2
Incubation3-5 daysRecommendation for infectionPlate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 31°C in a humidified 5% CO2 atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.Handling notesAntigenicity is specific for virus.Key abbreviations°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
Volume 提供形式 1.0 mL
Deposited asFeline rhinotracheitis virusDepositorsEV Davis, Norden Laboratories, Inc.Patent depositoryThis material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC. As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Type of isolateAnimalPatient age3 months