ATCC? Number:50197?
Organism: Singhamoeba horticola (Singh and Hanumaiah) Sawyer et al.
Designations: CR-1-3
Isolation: bottom sediment, Chester River, MD, 1985
Depositors: TK Sawyer
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 802: Sonneborn's Paramecium mediumTemperature: 25.0°C Duration: grown with Escherichia coli ATCC11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Comments: species description [20914]
References: 20914: Sawyer TK, et al. Singhamoeba horticala new combination Singh and Hanumaiah 1979 type species of Singhamoeba new genus. J. Protozool. 39: 107-109, 1992.