ATCC? Number:30815?
Organism: Leishmania tropica (Wright) Luhe
Designations: WHO Israel LRC 27
Isolation: lizard, Agama stellio, Tiberias, Israel, 1929
Depositors: J Janovy
History: ATCC<<--J Janovy<<--. . . <<--- A. David <<--- . .
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 811: Leishmania mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Comments: Taxonomy (ATCClab.). Promastigotes. Experimental infection of New World lizards [4861] Taxonomic status [23762] Variation in kinetoplast minicircle and maxicircle DNA sequences [24219] Cyclopropane fatty acid [24043]
References: 4861: Dollahon NR, Janovy J Jr.. Experimental infection of New World lizards with Old World lizard Leishmania species. Exp. Parasitol. 36: 253-260, 1974. PubMed: 4413377 23762: Simpson L. The status of Leishmania tarentolae/Trypanosoma platydactyli. Parasitol. Today 4: 115-118, 1988. 24043: Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 24219: Gomez-Eichelmann MC, et al. Comparison of several lizard Leishmania species and strains in terms of kinetoplast minicircle and maxicircle DNA sequences, nuclear chromosomes, and membrane lipids [published erratum appears in Mol. Biochem. Parasitol. 28: 301, 1988]. Mol. Biochem. Parasitol. 27: 143-158, 1988. PubMed: 3344003