ATCC® Number:30486™
Organism: Acanthamoeba polyphaga (Puschkarew) Page
Designations: Arabia Mountain
Isolation: soil, Arabia Mountain, DeKalb County, GA, 1972
Depositors: WS Jenkins
Biosafety Level:1
Shipped: dried
Growth Conditions: ATCCmedium 711: PYBTemperature: 25.0°C Duration: grown with Enterobacter aerogenes ATCC13048 Protocol: ATCCNO: 30011 SPEC: This strain is distributed as a dried preparation. See the general procedures for opening a dried vial. Aseptically add 1 ml of sterile distilled water to the inner shell vial, remove the filter paper aseptically with a pair of forceps, and place it in the center of an agar plate of ATCCmedium 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
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Comments: Indistinguishable from Acanthamoeba polyphaga ATCC30487 and very similar to A. griffini ATCC30731 on the basis of starch gel electrophoretic analysis of enzymes. Role of contractile vacuole in excystment [4594] phylogeny [24028]
Classification: KINGDOM: Protozoa
References: 4594: Jenkins WS. The role of the contractile vacuole in excystment of Acanthamoeba polyphaga (Puschkarew) Page. J. Protozool. 23: 24A, 1976. 23896: Daggett PM, et al. Distribution and possible interrelationships of pathogenic and nonpathogenic Acanthamoeba from aquatic environments. Microb. Ecol. 8: 371-386, 1982. 24028: Daggett PM, et al. A molecular approach to the phylogeny of Acanthamoeba. Biosystems 18: 399-405, 1985. PubMed: 4084681
ATCC30486
