ATCC? Number:VR-1602?
Classification: Adenoviridae, Mastadenovirus, Human adenovirus B
Agent: Human adenovirus 50
Strain: Wan [RIVM no. 88-1773)
Original Source: Isolated from a fecal sample from a human male suffering from diarrhea, Amsterdam, 1988.
Depositors: JC de Jong [44818]
Biosafety Level:2 [BSL 2 ]
Shipped: frozen
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Host Organism : Production Host: A549 (ATCCCCL-185) Alternate Host(s): Human cell cultures, GaBi, HEp-2
Incubation : For best results, plate cells 24-48 hours in advance. Infect at about 80% confluence using a multiplicity of infection (MOI) of 0.1 to 1.0. Incubate for 3 to 7 days at 37° C in a humidified atmosphere of 5% CO2 in air.
Effect : Cytopathic effects in culture include enlargement, rounding and clumping or roping of refractile cells.
Store at : -70°C or colder
Comments : Hemagglutinates Rhesus monkey RBCs.