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Chlamydia trachomatis 拉丁名

(ATCC? VR-886?) 統(tǒng)一編號

Classification Chlamydiaceae, Chlamydia Deposited As Chlamydia trachomatis (Busacca) Rake Agent Chlamydia trachomatis Strain Trachoma type J strain UW-36/Cx 

Biosafety Level 生物安全等級 2 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 1 mL per vial 

Storage Conditions 保藏方法 -70°C or colder 

Comments 注釋 FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth. 

Antigenically related but distinct from type C. 

Disrupt cells of seed stock using glass beads and vortex. 

Infect shell vial monolayer with 0.1 mL of diluted seed stock. contrifuge at 2000X for 1 hr. 

Feed with medium containing cycloheximide. This preparation has tested negative for Mycoplasma by culture; the PCR test results are ambiguous. 

Antigenically related but distinct from type C. 

Effect on Host CPE, intracellular inclusion bodies 

Recommended Host McCoy cells (ATCC? CRL-1696?) 

Growth Conditions 生長條件 

Temperature 培養(yǎng)溫度 : 36°C 

Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent. 

Incubation: 3 days, a 5% CO2 in air atmosphere is recommended Effect on Host CPE, intracellular inclusion bodies 

Name of Depositor 寄存人 SP Wang, CDC Source Human cervix, cervicitis, Seattle, 1971 Year of Origin 1971 

References 參考文獻 Wang S, Grayston JT. Chlamydia trachomatis immunotype J. J. Immunol. 115: 1711-1716, 1975. PubMed: 810514