ATCC? Number:VR-2001?
Classification: Herpesviridae, Mardivirus
Agent: Gallid herpesvirus 3 deposited as Marek's disease virus type 2
Strain: SB-1 (clone of SB)
Original Source: chicken (S-strain) peripheral blood lymphocytes
Depositors: BW Calnek
Biosafety Level:2
Shipped: frozen
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Host Organism : chicken embryo fibroblasts
Incubation : Max Temperature: 39.0°C Min Temperature: 38.0°C Duration: 7 days
Effect : Yes, in vitro effects: Cytopathic effects (syncytium formation, cell rounding)
Store at : Liquid nitrogen
Comments : Non-oncogenic, cell-associated. Will protect against MDV and non-virus producing JM tumor transplant. NOTE: This material is cited in a U.S. and/or other Patent Application and may not be used to infringe the patent claims. Note that SB-1 is strictly cell-associated, damage to the infected cells will cause loss of titer. Thaw frozen ampule in 27C waterbath; dilute content slowly in 9.0 ml medium or PBS; pellet cells (10min at 400Xg); resuspend in culture medium. Non-oncogenic, cell-associated. Will protect against MDV and non-virus producing JM tumor transplant.