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ATCC? Number:VR-1683?
Classification: Orthomyxoviridae, Influenzavirus A
Agent: Influenza A virus (H1N1)
Strain: A/Swine/Iowa/15/30 (TC-adapted)
Original Source: Swine in Iowa, 1930
Depositors: A. P. Kendal and ATCC
Biosafety Level:2
Shipped: frozen
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Host Organism : Production Host: MDCK (ATCCCCL-34) Alternate Hosts: Chicken embryo, MDCK and Monkey kidney cells (MkK)
Incubation : For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33.5℃ in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCCVR-1683 should be grown in serum free media that contains 1 ug/ml TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Effect : CPE (cell rounding, degeneration, sloughing)
Store at : -70℃ or colder
Comments : Derived by adaptation of egg-passage A/Swine/Iowa/15/30 (ATCCVR-333) to MDCK (ATCCCCL-34) cells. (Note: ATCCVR-333 and ATCCVR-1683 have not been compared with respect to sequence or infectivity in chick embryo and tissue culture).