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Chlamydomonas reinhardtii Dangeard菌株拉丁名

(ATCC? 18798?)菌株編號(hào)

Strain Designations菌株別名 1 wild type

Application Control of flagellar motion

Biosafety Level生物安全等級(jí) 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation分離源 Japan

Product Format提供形式 frozen

Storage Conditions Frozen保藏條件 Cultures: -70℃ for 1 week; liquid N2 vapor for long term  storage

Freeze-dried 凍干物Cultures: 2-8℃

Live Cultures活菌: See Protocols section for handling information

Type Strain模式菌株 yes

Comments注釋  The effect of temperature, Ca2+, Mg2+, and Ni2+ ions on the swimming speed Cinematographic investigation of motility Cinematographical comparison of motility Control of flagellar motion Photosynthetic

Medium ATCC? Medium 5: Sporulation agar

Growth Conditions生長(zhǎng)條件

Temperature培養(yǎng)溫度: 20℃ to 25℃

Culture System培養(yǎng)系統(tǒng): Axenic Cryopreservation Harvest and Preservation Harvest cells from a culture which is at or near peak density by adding 3.0 mL fresh

ATCC medium 5 broth to the slant and washing cells into suspension.

Adjust the concentration of cells to 4 x 106/mL with fresh broth medium, then dilute to half this concentration by adding an equal amount of a 10% (v/v) sterile methanol solution in fresh ATCC medium 5 broth.

Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

The time from mixing of the cell preparation and the methanol solution, before the cooling cycle begins, should be no greater than 15 min.

Place vials in a controlled rate freezing unit. From room temperature cool at -1℃/min to -40℃.

If freezing unit can compensate for the heat of fusion, maintain rate at -1℃/min through heat of fusion.

At -40℃ plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1℃ freezing apparatus.

Place the apparatus at -80℃ for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.

(The cooling rate in this apparatus is approximately -1℃/min.) The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator.

Frozen preparations stored below -130℃ are stabile indefinitely.

Those stored at temperatures above -130℃ are progressively less stabile as the storage temperature is elevated. Vials can be stored between -80℃ and -70℃ for no longer than one week.

To establish a culture from the frozen state place an ampule in a water bath set at 35℃.

Immerse the vial to a level just above the surface of the frozen material.

Do not agitate the vial. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and add to a centrifuge tube containing 5 mL of ATCC medium 5 without agar. Centrifuge at 300 x g for 5 min.

Remove most of the supernatant (methanol, which can inhibit growth) and then resuspend the pellet.

Transfer the culture to a fresh tube of ATCC medium 5 and incubate on a 15° horizontal slant at 50 to 100 ?Einsteins/m2/s irradiance at 25℃ with the cap loosened one half turn. Maintain under a 14/10 h light-dark photoperiod.

Name of Depositor M Nishimura

References參考文獻(xiàn)  Racey TJ, Hallett FR. A quasi-elastic light scattering and cinematographical comparison of three strains of motile Chlamydomonas reinhardtii: a wild type strain, a colchicine resistant mutant and a backward swimming mutant. J. Muscle Res. Cell Motil. 4: 333-351, 1983. PubMed: 6874918

Racey TJ, et al. A quasi-elastic light scattering and cinematographic investigation of motile Chlamydomonas reinhardtii. Biophys. J. 35: 557-571, 1981. PubMed: 7272452

Racey TJ, Hallett FR. The effect of temperature, Ca2+, Mg2+ and Ni2+ ions on the swimming speed of C. reinhardii determined by quasi-elastic light scattering. Exp. Cell Res. 136: 371-378, 1981. PubMed: 7308314

Racey TJ, Hallett FR. A low angle quasi-elastic light scattering investigation of Chlamydomonas reinhardtii. J. Muscle Res. Cell Motil. 4: 321-331, 1983. PubMed: 6874917

Nichols KM, Rikmenspoel R. Control of flagellar motion in Chlamydomonas and Euglena by mechanical microinjection of Mg2+ and Ca2+ and by electric current injection. J. Cell Sci. 29: 233-247, 1978. PubMed: 415066