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Clostridium difficile (Hall and O'Toole) Prevot 拉丁名

(ATCC? 700057?) 編號

Strain Designations 菌株別名 VPI 11186 [6] 

Application 用途 Susceptibility testing 

Biosafety Level 生物安全等級 2 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 freeze-dried 

Storage Conditions 培養(yǎng)條件 

Frozen 冷凍物 : -80°C or colder 

Freeze-Dried 凍干物：2°C to 8°C 

Live Culture 活菌 :See Propagation Section 

Preceptrol? no 

Type Strain 模式菌株 no  

Comments 注釋 Toxinotype tcdA-, tcdB- 

Ribotype 038 

Binary toxin gene cdtB not amplified by PCR 

Nontoxigenic 

Medium 培養(yǎng)基 ATCC? Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)

ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood 

Growth Conditions 生長條件 

Temperature 培養(yǎng)溫度: 37°C 

Atmosphere 需氧情況 : Anaerobic gas mixture, 80% N2-10% CO2-10% H2 

Name of Depositor 寄存人 LV Moore 

Special Collection NCRR Contract 

Chain of Custody 來源國家 ATCC <-- LV Moore <-- H Beerens 6 

References 參考文獻 Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M11-A7. 

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 

BD GeneOhm Cdiff. BD Becton Dickinson.

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile 

Ribotyping Network for England and N. Ireland (CDRN). An 

enhanced DNA fingerprinting service to investigate potential 

Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986