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Bipolaris zeicola (Stout) Shoemaker, anamorph 拉丁名

(ATCC? 20154?) 統(tǒng)一編號(hào)

Deposited As Helminthosporium sp. 

Strain Designations 別名 149 [215] 

Alternate State Cochliobolus carbonum Nelson, teleomorph

Application  用途 produces methyl ester of 6'-ethyl-2',3',6',7',8',8'-alpha-hexa-hydro-4-methoxy-2-(methoxymethylene)-3-oxospiro(indoline-2,1'(5'H) indolizine)-7'-acetic acid 

Biosafety Level 生物安全等級(jí) 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 

Storage Conditions 保藏條件

Frozen 冷凍物: -80°C or colder 

Freeze-Dried 凍干物: 2°C to 8°C

Live Culture: See Propagation Section

Type Strain 模式菌種 no 

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. 

Preceptrol? no 

Comments 注釋 The strain was re-identified by DNA barcode sequencing 

Morphology On Czapek’s agar medium at 25°C after 6 days, mycelium gray becoming black, cob-web to cottony texture. Reverse black. Hyphae both hyaline and dematiacous, guttulate, thick-walled. Conidia yellow brown to reddish brown, obclavate, 3-4 septate, 22-37.5 X 10.5-37.5 μm.

Medium 培養(yǎng)基 ATCC? Medium 312: Czapek's agar

Growth Conditions 生長(zhǎng)條件

Temperature 培養(yǎng)溫度: 24°C to 26°C 

Atmosphere 需氧情況: Typical aerobic

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATCATTACACA

ACAAAATATGAAGGCCCTGGCTTCGCGGCCGGCTGAAATATTTTTTCACCCATGTCT

TTTGCGCACTTGTTGTTTCCTGGGCGGGTTTGCCCGCCACCAGGACCAAACCATAAA

CCTTTTTTTTTATGCAGTTACCATCAGCGTCAGTAAAAACAATGTAATTAATTACAA

CTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGC

GATACGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACAT

TGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTCGAGCGTCATTTGTACCTTCAA

GCTTTGCTTGGTGTTGGGCGTTTTTGTCTCCCTCTTTCTGGGAGACTCGCCTTAAAAC

GATTGGCAGCCGGCCTACTGGTTTCGGAGCGCAGCACATAATTTGCGCTTTGTATC

AGGAGAAAAGGACGGTAATCCATCAAGACTCTAGATTTTTAACTTTTGACCTCGGA

TCAGGTAGGGATACCCGCTGAACTTAAGCATATCAA 

D1D2 region of the 28S ribosomal RNA gene 

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGG

CGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTCTTTCAGAGTCCGAGTTGTAA

TTTGCAGAGGGCGCTTTGGCTTTGGCAGCGGTCCAAGTTCCTTGGAACAGGACGTCAC

AGAGGGTGAGAATCCCGTACGTGGTCGCTAGCTATTGCCGTGTAAAGCCCCTTCGAC

GAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTA

AATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCA

CTTTGGAAAGAGAGTCAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCAGC

CAGACTTGCTTGCAGTTGCTCATCCGGGCTTTTGCCCGGTGCACTCTTCTGTAGGCA

GGCCAGCATCAGTTTGGGCGGTGGGATAAAGGTCTCTGTCACGTACCTCTCTTCGG

GGAGGCCTTATAGGGGAGACGACATACCACCAGCCTAGACTGAGGTCCGCGCAT

CTGCTAGGATGCTGGCGTAATGGCTGTAAGCGGC 

Name of Depositor Smith, Kline & French Labs. 

U.S. Patent Number 3,534,052 

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Chain of Custody ATCC <-- Smith, Kline & French Labs. <-- Rutgers Univ. 215

References Douglas B, et al. Methyl ester of 6'-ethyl-2',3',6',7',8',8'a-hexahydro-4-methoxy-alpha-(methoxy-me thylene)-3-oxospiro[indoline-2,1'(5'-H)-indolizine]-7'-acetic acid. US Patent 3,534,052 dated Oct 13 1970