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平臺(tái)編號(hào)：bio-134930
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細(xì)胞信息： Panc 08.13
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：STR鑒定正確
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
人胰腺癌細(xì)胞
信息
名稱(chēng) Panc 08.13 (人胰腺癌細(xì)胞) (STR鑒定正確)
別稱(chēng) Panc 8.13; Panc-08.13; PANC-08-13; Panc_08_13; Panc08.13; Panc8.13; Panc-8_13; Panc-813; PANC 813; Panc813; PANC813; PANC0813; Panc0813; Pa14C; PL9; PL-9
種屬 人
生長(zhǎng)特性 貼壁細(xì)胞
細(xì)胞形態(tài) 上皮細(xì)胞樣
生長(zhǎng)培養(yǎng)基 RPMI-1640＋15% FBS＋10 IU/mL Insulin ＋1% P/S
凍存條件 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度：液氮
培養(yǎng)條件 氣相：空氣，95%；CO2，5%溫度：37℃
推薦傳代比例 1:2-1:4
推薦換液頻率 2-3次/周
參考資料(來(lái)源文獻(xiàn))
背景描述 Panc 08.13 is an epithelial cell line that was isolated in 1995 from the pancreas of a White, 85-year-old, male patient with adenocarcinoma. This cell line was deposited by EM Jaffee and can be used in cancer research.The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. The cells have a reported plating efficiency of 40%.
年齡（性別） 男性，85歲
組織來(lái)源 胰腺
細(xì)胞類(lèi)型 腫瘤細(xì)胞
腫瘤類(lèi)型 胰腺癌細(xì)胞
生物安全等級(jí) 1
倍增時(shí)間 20.0 hours (PubMed=9612602); 117 hours (PubMed=25984343)
致瘤性 Yes; Yes, forms tumors in nude or SCID mice
抗原表達(dá)情況 MHC class I +; MHC class II -
基因表達(dá)情況 cytokeratin 7; cytokeratin 18
保藏機(jī)構(gòu) ATCC; CRL-2551
STR鑒定
STR位點(diǎn)信息
Amelogenin X
CSF1PO 11
D2S1338 17,18
D3S1358 16
D5S818 13,14
D7S820 11
D8S1179 10,14
D13S317 13
D16S539 13
D18S51 19
D19S433 13
D21S11 32.2
FGA 21,23
PentaD 10
PentaE 16,17
TH01 6,9.3
TPOX 8,12
vWA 18
D6S1043 11,14
D12S391 23
D2S441 10,11.3
名稱(chēng) Panc 08.13 (人胰腺癌細(xì)胞) (STR鑒定正確)
別稱(chēng) Panc 8.13; Panc-08.13; PANC-08-13; Panc_08_13; Panc08.13; Panc8.13; Panc-8_13; Panc-813; PANC 813; Panc813; PANC813; PANC0813; Panc0813; Pa14C; PL9; PL-9
種屬 人
生長(zhǎng)特性 貼壁細(xì)胞
細(xì)胞形態(tài) 上皮細(xì)胞樣
生長(zhǎng)培養(yǎng)基 RPMI-1640＋15% FBS＋10 IU/mL Insulin ＋1% P/S
凍存條件 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度：液氮
培養(yǎng)條件 氣相：空氣，95%；CO2，5%溫度：37℃
推薦傳代比例 1:2-1:4
推薦換液頻率 2-3次/周
參考資料(來(lái)源文獻(xiàn))
背景描述 Panc 08.13 is an epithelial cell line that was isolated in 1995 from the pancreas of a White, 85-year-old, male patient with adenocarcinoma. This cell line was deposited by EM Jaffee and can be used in cancer research.The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. The cells have a reported plating efficiency of 40%.
年齡（性別） 男性，85歲
組織來(lái)源 胰腺
細(xì)胞類(lèi)型 腫瘤細(xì)胞
腫瘤類(lèi)型 胰腺癌細(xì)胞
生物安全等級(jí) 1
倍增時(shí)間 20.0 hours (PubMed=9612602); 117 hours (PubMed=25984343)
致瘤性 Yes; Yes, forms tumors in nude or SCID mice
抗原表達(dá)情況 MHC class I +; MHC class II -
基因表達(dá)情況 cytokeratin 7; cytokeratin 18
保藏機(jī)構(gòu) ATCC; CRL-2551
STR鑒定
STR位點(diǎn)信息
Amelogenin X
CSF1PO 11
D2S1338 17,18
D3S1358 16
D5S818 13,14
D7S820 11
D8S1179 10,14
D13S317 13
D16S539 13
D18S51 19
D19S433 13
D21S11 32.2
FGA 21,23
PentaD 10
PentaE 16,17
TH01 6,9.3
TPOX 8,12
vWA 18
D6S1043 11,14
D12S391 23
D2S441 10,11.3
