Synonyms: UPCI-SCC-131
Background: established from an oral squamous cell carcinoma of a 73-year-old Caucasian man; the cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-412-648-4004) at the University of Pittsburgh, Office of Technology Management
Species: human (Homo sapiens)
Tissue: N/A
Disease: squamous cell carcinoma
Gender: N/A
Morphology: epithelial cells growing adherently as monolayer
Growth Mode: N/A
Doubling Time: ca. 50-60 hours
DNA Profile: N/A
Cell Line Authentication Service
Culture Medium: MEM + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% FBS
We strongly suggest to purchase the complete medium from
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+)
Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile
Species: N/A
Cytogenetics: N/A
Virus PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV -