細(xì)胞名稱:D3小鼠胚胎干細(xì)胞D3
描述:小鼠全能性胚胎干細(xì)胞,每支細(xì)胞量1×106
動(dòng)物種別:小鼠,129/Sv+c/+p品系
性別:
組織來(lái)源:小鼠囊胚內(nèi)細(xì)胞團(tuán)
形態(tài):球形克隆
培養(yǎng)基和添加劑:mES完全培養(yǎng)液配方(600 ml): DMEM (Invitrogen 12430) 497 ml ES級(jí)FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl(終濃度為1000U/ml) β-Mer (Invitrogen 21985) 1.5 ml 氣相:空氣,95%;二氧化碳,5%。溫度:37攝氏度。
供應(yīng)限制:A。僅供研究之用。
REFERENCE:Doetschman TC, et al. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J. Embryol. Exp. Morphol. 87: 27-45, 1985. PubMed: 3897439 Williams RL, et al. Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336: 684-687, 1988. PubMed: 3143916 Gossler A, et al. Transgenesis by means of blastocyst-derived embryonic stem cell lines. Proc. Natl. Acad. Sci. USA 83: 9065-9069, 1986. PubMed: 3024164 Doetschman T, et al. Targeted mutation of the Hprt gene in mouse embryonic stem cells. Proc. Natl. Acad. Sci. USA 85: 8583-8587, 1988. PubMed: 3186749 Chambon P, et al. Genetically engineered mice containing alterations in the genes encoding retinoic acid receptor proteins. US Patent 6,486,381 dated Nov 26 2002
中文名稱:小鼠胚胎干細(xì)胞D3
提供形式:復(fù)蘇狀態(tài)