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G-401 [G401]

平臺編號：bio-68483
國際編號：ATCCCRL-1441
細(xì)胞信息： G-401 [G401]
規(guī)格：frozen
用途：ATCC原裝細(xì)胞
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）

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G-401 [G401] (ATCC? CRL-1441?)

OrganismHomo sapiens, human

Tissue

kidney

Product Formatfrozen

Morphologyepithelial

Culture Propertiesadherent

Biosafety Level1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Diseaserhabdoid tumor (formerly classified as Wilms' tumor)

Age3 months

Gendermale

EthnicityCaucasian

Storage Conditionsliquid nitrogen vapor temperature

KaryotypeThe cell line has the 46, XY karyotype. There are no apparent marker chromosomes. The rate of polyploidy was 2.8%. The presence of the Y chromosome was confirmed in Q-band observation.

DerivationG-401 was deposited as a cell line derived from a Wilms' tumor.

Clinical Data

3 months

Caucasian

male

Genes Expressednephroblast growth factor (NB-GF)

Cellular Products

nephroblast growth factor (NB-GF)

TumorigenicYes

Effects

Yes, forms colonies in soft agar

Comments

G-401 was deposited as a cell line derived from a Wilms' tumor. Due to a change in the classification of such tumors, the cell line was examined by Garvin et al. and found to be more appropriately classified as derived from a rhabdoid tumor of the kidney. Ref

Complete Growth MediumThe base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37℃) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation

Freeze Medium: Complete growth medium, 95%; DMSO, 5%

Storage Temperature: Liquid nitrogen vapor temperature

Culture Conditions

Temperature: 37℃

STR Profile

Amelogenin: X,Y

CSF1PO: 11,13

D13S317: 9,14

D16S539: 12

D5S818: 13

D7S820: 11,14

THO1: 8,9.3

TPOX: 8,11

vWA: 16

Isoenzymes

G6PD, B

Name of DepositorPT Peebles

References

Weissman BE, et al. Introduction of a normal human chromosome 11 into a Wilms' tumor cell line controls its tumorigenic expression. Science 236: 175-176, 1987. PubMed: 3031816

Burrow CR, Wilson PD. A putative Wilms tumor-secreted growth factor activity required for primary culture of human nephroblasts. Proc. Natl. Acad. Sci. USA 90: 6066-6070, 1993. PubMed: 8392186

Karnieli E, et al. The IGF-1 receptor gene promoter is a molecular target for the Ewing's Sarcoma=Wilms' Tumor 1 fusion protein. J. Biol. Chem. 271: 19304-19309, 1996. PubMed: 8702614

Garvin AJ, et al. The G401 cell line, utilized for studies of chromosomal changes in Wilms' tumor, is derived from a rhabdoid tumor of the kidney. Am. J. Pathol. 142: 375-380, 1993. PubMed: 8382007

Cross References

Nucleotide (GenBank) : D10232 Homo sapiens mRNA for renin-binding protein, complete cds.

Source: Organ: kidney
Disease: rhabdoid tumor (formerly classified as Wilms' tumor)
Cellular Products:nephroblast growth factor (NB-GF)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 9,14
D16S539: 12
D5S818: 13
D7S820: 11,14
THO1: 8,9.3
TPOX: 8,11
vWA: 16
Cytogenetic Analysis:The cell line has the 46, XY karyotype. There are no apparent marker chromosomes. The rate of polyploidy was 2.8%. The presence of the Y chromosome was confirmed in Q-band observation.
Isoenzymes: G6PD, B
Age: 3 months
Gender: male
Ethnicity: Caucasian
Comments:G-401 was deposited as a cell line derived from a Wilms' tumor. Due to a change in the classification of such tumors, the cell line was examined by Garvin et al. and found to be more appropriately classified as derived from a rhabdoid tumor of the kidney. [49833 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0℃
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
References: 22955: Weissman BE, et al. Introduction of a normal human chromosome 11 into a Wilms' tumor cell line controls its tumorigenic expression. Science 236: 175-176, 1987. PubMed: 3031816
23338: Burrow CR, Wilson PD. A putative Wilms tumor-secreted growth factor activity required for primary culture of human nephroblasts. Proc. Natl. Acad. Sci. USA 90: 6066-6070, 1993. PubMed: 8392186
33153: Karnieli E, et al. The IGF-1 receptor gene promoter is a molecular target for the Ewing's Sarcoma=Wilms' Tumor 1 fusion protein. J. Biol. Chem. 271: 19304-19309, 1996. PubMed: 8702614
49833: Garvin AJ, et al. The G401 cell line, utilized for studies of chromosomal changes in Wilms' tumor, is derived from a rhabdoid tumor of the kidney. Am. J. Pathol. 142: 375-380, 1993. PubMed: 8382007