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平臺(tái)編號(hào)：bio-69260
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國際編號(hào)：CRL-2392?
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細(xì)胞信息： GA-10
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
GA-10
生長狀態(tài)：懸浮生長
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
ATCC Number：CRL-2392?
相關(guān)疾病：淋巴瘤
數(shù)量：大量
細(xì)胞類型：B淋巴細(xì)胞
年限：41 years
是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：50 次 Designations: GA-10
Depositors: ?AP Rapoport
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens deposited as human
Morphology:lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin; kappa light chain
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD10 +, CD19 +, CD20 +, CD40 + (10%), CD45 +, CD77 + (90%), CD34 -
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,20
Cytogenetic Analysis:The reported karyoype was 46, XY, dup(1) (pter to q32::q21 to qter), t(8;14) (q24.1; q32), -10, +der(10)t(Y;10)(q12:q26), -21, +der (21) t (?; 21) (?; p11)., In addition a few cells lacked a second chromosome 21 and carried an additional der(21) t(?;21) (?;q22). The Vh segment in this cell line was Vh4-34 (Vh 4.21).
Age: 41 years
Gender: male
Ethnicity: Caucasian, White
Comments:GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.
The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy).
The cells express relatively high levels of CD77 (approximately 90%); CD77 is also known as Burkitt's lymphoma antigen (BLA).
GA-10 cells exhibit sensitivity to Shiga Toxin (Stx) in toxicity studies.
This cell line is slightly more sensitive to doxorubicin and etoposide than HL-60 leukemia cells.
Flavopiridol, a potent inhibitor of cell cycle progression through G1 and G2, produces apoptosis of the GA-10 cells at relatively low concentrations of 20-30 nM.
GA-10 cells are negative for Epstein-Barr virus (EBV).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 24 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derived from same cell line:ATCC CRL-2393
derived from same cell line:ATCC CRL-2394
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
ATCC Number：CRL-2392?
相關(guān)疾病：淋巴瘤
數(shù)量：大量
細(xì)胞類型：B淋巴細(xì)胞
年限：41 years
是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：50 次 Designations: GA-10
Depositors: ?AP Rapoport
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens deposited as human
Morphology:lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin; kappa light chain
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD10 +, CD19 +, CD20 +, CD40 + (10%), CD45 +, CD77 + (90%), CD34 -
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,20
Cytogenetic Analysis:The reported karyoype was 46, XY, dup(1) (pter to q32::q21 to qter), t(8;14) (q24.1; q32), -10, +der(10)t(Y;10)(q12:q26), -21, +der (21) t (?; 21) (?; p11)., In addition a few cells lacked a second chromosome 21 and carried an additional der(21) t(?;21) (?;q22). The Vh segment in this cell line was Vh4-34 (Vh 4.21).
Age: 41 years
Gender: male
Ethnicity: Caucasian, White
Comments:GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.
The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy).
The cells express relatively high levels of CD77 (approximately 90%); CD77 is also known as Burkitt's lymphoma antigen (BLA).
GA-10 cells exhibit sensitivity to Shiga Toxin (Stx) in toxicity studies.
This cell line is slightly more sensitive to doxorubicin and etoposide than HL-60 leukemia cells.
Flavopiridol, a potent inhibitor of cell cycle progression through G1 and G2, produces apoptosis of the GA-10 cells at relatively low concentrations of 20-30 nM.
GA-10 cells are negative for Epstein-Barr virus (EBV).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml.
Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 24 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derived from same cell line:ATCC CRL-2393
derived from same cell line:ATCC CRL-2394
