細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：1
物種來源：人
年限：18 years
ATCC Number：HTB-86?
器官來源：骨
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運輸方式：凍存運輸
數(shù)量：大量
規(guī)格：60 ml Designations: SK-ES-1
Depositors: ?G Trempe, LJ Old
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Organism: Homo sapiens
Morphology:epithelial
Source: Organ: bone
Disease: sarcoma (anaplastic osteosarcoma or Ewing's sarcoma)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Isolation: Isolation date: 1971
Tumorigenic:Yes
Antigen Expression:Blood Type A; Rh+
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,9
D16S539: 11
D5S818: 12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8
vWA: 14,17
Cytogenetic Analysis:modal number = 49; range = 44 to 51. This is a hyperdiploid human cell line with the modal chromosome number of 49 occurring in 34% of cells. However, cells with 50 chromosomes also occurred at high percentage (24%). At least 11 markers were consistently present in most cells. Among them were: der(3)t(3;12) (q21;q13); del(3) (q21); inv(1) (p36.1q21), and der(16)t(5;16) (q13;p13.3), all present in single copy per cell. At least three Y-autosome markers were detected. Single normal X chromosome was found in every cell. Normal Y chromosome was not found.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 1-2
Age: 18 years
Gender: male
Ethnicity: Caucasian
Comments:Antibodies to the sarcoma lines were detected in sera of patients with sarcomas after absorption with non-sarcoma lines. Sera from normal donors were also cytotoxic.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.
22423: Bloom ET. Further definition by cytotoxicity tests of cell surface antigens of human sarcomas in culture. Cancer Res. 32: 960-967, 1972. PubMed: 4502173
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
