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平臺編號：bio-69346
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國際編號：HTB-62?
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細(xì)胞信息： P3HR-1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
P3HR-1
數(shù)量：大量
細(xì)胞類型：B淋巴細(xì)胞
年限：7 years
是否是腫瘤細(xì)胞：1
物種來源：人
生長狀態(tài)：懸浮生長
ATCC Number：HTB-62?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。毫馨土?br /> 細(xì)胞形態(tài)：淋巴樣
組織來源：ascites Designations: P3HR-1
Depositors: ?G Trempe, LJ Old
Biosafety Level:2 [Cells Contain Herpesvirus ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Tissue: ascites
Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19
Isoenzymes: ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 2
PGM1, 1
Age: 7 years
Gender: male
Ethnicity: Black, African
Comments:The P3HR-1 line is a clonally derived subline of Jiyoye (ATCC CCL-87 ) which yielded up to 50-85% immunofluorescent positive cells when stained with selected human sera.
Herpes type virus particles (Epstein-Barr virus) were observed in as many as 78% of the cells examined with the electron microscope and a good correlation between presence of viral particles and immunofluorescence was obtained.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 X 10 exp5 cells/ml and maintain at between 4 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:derivative:ATCC CCL-87
References: 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763
22549: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722
22760: . . Sci. Rep. Res. Inst. Tohoku Univ. Ser. C Med. 16: 97-101, 1969.
22816: Giraldo G, et al. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454-478, 1971. PubMed: 4329447
22878: Hinuma Y, et al. Immunofluorescence and herpes-type virus particles in the P3HR-1 Burkitt lymphoma cell line. J. Virol. 1: 1045-1051, 1967. PubMed: 4912237
23216: Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014
細(xì)胞類型：B淋巴細(xì)胞
年限：7 years
是否是腫瘤細(xì)胞：1
物種來源：人
生長狀態(tài)：懸浮生長
ATCC Number：HTB-62?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。毫馨土?br /> 細(xì)胞形態(tài)：淋巴樣
組織來源：ascites Designations: P3HR-1
Depositors: ?G Trempe, LJ Old
Biosafety Level:2 [Cells Contain Herpesvirus ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Tissue: ascites
Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19
Isoenzymes: ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 2
PGM1, 1
Age: 7 years
Gender: male
Ethnicity: Black, African
Comments:The P3HR-1 line is a clonally derived subline of Jiyoye (ATCC CCL-87 ) which yielded up to 50-85% immunofluorescent positive cells when stained with selected human sera.
Herpes type virus particles (Epstein-Barr virus) were observed in as many as 78% of the cells examined with the electron microscope and a good correlation between presence of viral particles and immunofluorescence was obtained.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 X 10 exp5 cells/ml and maintain at between 4 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:derivative:ATCC CCL-87
References: 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763
22549: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722
22760: . . Sci. Rep. Res. Inst. Tohoku Univ. Ser. C Med. 16: 97-101, 1969.
22816: Giraldo G, et al. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454-478, 1971. PubMed: 4329447
22878: Hinuma Y, et al. Immunofluorescence and herpes-type virus particles in the P3HR-1 Burkitt lymphoma cell line. J. Virol. 1: 1045-1051, 1967. PubMed: 4912237
23216: Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014
