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細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
ATCC Number：CRL-2422?
相關(guān)疾病：腺癌
年限：63 years old adult
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
器官來(lái)源：前列腺 Designations: MDA PCa 2b
Depositors: ?NM Navone
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:epithelial


Source: Organ: prostate
Disease: adenocarcinoma
Derived from metastatic site: bone
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:androgen receptor, expressed
Tumorigenic:Yes
Antigen Expression:prostate specific antigen (PSA)
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 10,11
D5S818: 12,13
D7S820: 8,10
THO1: 8,9
TPOX: 8,11
vWA: 16
Age: 63 years old adult
Gender: male
Ethnicity: Black
Comments:MDA PCa 2b was established from a bone metastasis of 63 year old Black male with androgen-independent adenocarcinoma of the prostate.
The cell line expresses prostate specific antigen (PSA) and androgen receptor, grows in vitro and in vivo, and is androgen sensitive.
Cells from this cell line produce tumors in nude mice when injected either subcutaneously or orthotopically (intraprostatic).
This cell line is suitable for studying cell growth regulation by androgens.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:

• fetal bovine serum to a final concentration of 20%
• 25 ng/ml cholera toxin
• 10 ng/ml mouse Epidermal Growth Factor
• 0.005 mM phosphoethanolamine
• 100 pg/ml hydrocortisone
• 45 nM selenious acid
• 0.005 mg/ml bovine insulin

Do not filter complete medium.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
6. Place culture vessels in incubators at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
derived from same individual:ATCC CRL-2421
purified DNA:ATCC CRL-2422D
References: 40479: Navone NM, et al. Establishment of two human prostate cancer cell lines derived from a single bone metastasis. Clin. Cancer Res. 3: 2493-2500, 1997. PubMed: 9815652