啟動子: Tac/lac
復(fù)制子: ColE1 ori
質(zhì)粒分類: 大腸桿菌載體；PGEX系列表達(dá)質(zhì)粒
質(zhì)粒大小: 4984bp
質(zhì)粒標(biāo)簽: N-GST，N-PP/3C
原核抗性: 氨芐青霉素Amp
克隆菌株: DH5α
培養(yǎng)條件: 37℃，有氧，LB
表達(dá)宿主: 大腸桿菌
誘導(dǎo)方式: IPTG或乳糖及其類似物
5'測序引物: pGEX5: GGGCTGGCAAGCCACGTTTGGTG
3'測序引物: pGEX3: CCGGGAGCTGCATGTGTCAGAGG
備注: 可用GST親和柱純化重組蛋白
質(zhì)粒簡介
There are a range of Gluthatione Sepharose? prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 μl use GST SpinTrap? microspin columns or GST MultiTrap? 4B 96-well plates. For sample volumes between 600 μl and 10 ml use GST GraviTrap? gravity flow column. Where sample volumes are above 10 ml, use LabMate? reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap? 1 and 5 ml columns and GST HiPrep? FF 16/10 column are available for purification in a chromatography system such as the ?KTA? design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty gravity flow columns with required buffers. For simplified buffer preparation use the GST Buffer Kit. Ordering information for all associated products is listed below.
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