擬桿菌綱的種類很多生活在人或者動物的腸道中,有些時候成為病原菌。在糞便中,以細胞數(shù)目計,擬桿菌屬(Bacteroides)是主要微生物種類。
一、菌種簡介
平臺編號:Bio-79895
規(guī)格:Freeze-Dried
拉丁屬名:Bacteroides Stercoris
菌株名稱:糞便擬桿菌
用途:ATCC原裝進口
注意事項:僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準)
二、詳細描述
Bacteroides stercoris Johnson et al. 拉丁名
(ATCC? 43183?) 統(tǒng)一編號
Strain Designations 菌種別名 : VPI B5-21 [CIP 104203T, JCM 9496, subsp. a] /
Type Strain 模式菌種: yes /
Biosafety Level 生物安全等級 : 2
Application 用途 Characterization Isolation Human feces, Texas
Biosafety Level 生物安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏方法
Frozen(冷凍物): -80℃ or colder
Freeze-Dried(凍干物): 2℃ to 8℃
Live Culture(活物): See Propagation Section
Preceptrol? no Genome Sequenced Strain Yes
Type Strain 模式菌種 yes
Comments 注釋 This strain has been sequenced as a reference genome for the NIH Human Microbiome Project.
Medium 培養(yǎng)基 ATCC? Medium 1490: Modified chopped meat medium
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度: 37℃
Atmosphere 需氧情況 : Anaerobic gas mixture, 80% N2-10% CO2-10% H2
Name of Depositor 寄存人 LV Moore
Chain of Custody 來源國家 ATCC <-- LV Moore <-- W.E.C. Moore
Isolation 分離基物 Human feces, Texas
References 參考文獻 Johnson JL. Bacteroides caccae sp. nov., Bacteroides merdae sp. nov., and Bacteroides stercoris sp. nov. isolated from human feces. Int. J. Syst. Bacteriol. 36: 499-501, 1986.
Johnson JL. Taxonomy of Bacteroides I. Deoxyribonucleic acid homologies among Bacteroides fragilis and other saccharolytic Bacteroides species. Int. J. Syst. Bacteriol. 28: 245-256, 1978.
Johnson JJ, Ault DA. Taxonomy of the bacteroides II. Correlation of phenotypic characteristics with deoxyribonucleic acid homology groupings for Bacteroides fragilis and other saccharolytic Bacteroides species. Int. J. Syst. Bacteriol. 28: 257-268, 1978.
Cross References
Nucleotide (GenBank) : X83953 B.stercoris 16S rRNA (ATCC 43183T).
Nucleotide (GenBank) : U50417 Bacteroides stercoris 16S ribosomal RNA gene, partial sequence.
Nucleotide (GenBank) : ABFZ02000000 Bacteroides stercoris ATCC 43183, whole genome shotgun sequencing project。
三、菌株形態(tài)
菌落直徑為1-2mm,圓形,邊緣整齊,不透明,正面白色,中間凸起,表面光滑,表面明亮,質(zhì)地濕潤,易挑起,G-(紅),桿菌。
四、傳代方法
①準備上述平板 1-2 塊;
②平板表面消毒后在安全柜中打開;
③吸取 1-3ml 無菌水(置于厭氧環(huán)境平衡24h)打入平板中,用無菌涂布棒在平板表層來回刮取菌苔,制成菌懸液;
④用巴氏吸管吸取菌懸液,打入到新鮮的平板上,涂布均勻;
⑤將平板置于上述培養(yǎng)條件下培養(yǎng)即可。
微生物菌種查詢網(wǎng)作為中國微生物菌種中心引航者,單位代為引進美國ATCC微生物菌種保藏中心,德國DSMZ菌種保藏中心,荷蘭CBS菌種保藏中心,日本JCM微生物菌種保藏中心,包括其中的ATCC微生物細菌與噬菌體(18382);細胞系和雜交瘤(4997);真菌和酵母(58183);原代細胞(146);原生動物和藻類(2428);干細胞(102);載體,克隆與分子(18006);病毒(3594);核酸 - DNA / RNA(1350),引進進口微生物菌種貨期短,質(zhì)量保證,UPS國際快遞運輸。