ATCC? Number:30258?
Organism: Crithidia luciliae (Strickland) Wallace and Clark
Designations: [ATCC14765]
Isolation: green bottle fly, Phaenicia sericata, Minneapolis, MN, 1958
Depositors: FG Wallace, T Clark
Biosafety Level:1
Shipped: frozen
Growth Conditions: ATCCmedium 1011: Diphasic blood agar mediumATCCmedium 355: Crithidia mediumTemperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
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Type Strain: yes [4616]
Applications: detection of lupus erythematosus [24182] assay of unconjugated pteridines; listed here as ATCC14765 [5137]
Comments: Flagellate parasites from a fly [4616] Effects of purine starvation [21692] Riboprinting and taxonomy [23607] Thermal regulation of oxygen-scavenging enzymes [23820] Multiple distinct site-specific elements in miniexon arrays [24177] Cyclopropane fatty acid [24043]
References: 4616: Wallace FG, Clark TB. Flagellate parasites of the fly, Phaenicia sericata (Meigen). J. Protozool. 6: 50-61, 1959. 4825: Faria e Silva PM, et al. Herpetomonas roitmani (Fiorini et al., 1989) n. comb.: a trypanosomatid with a bacterium-like endosymbiont in the cytoplasm. J. Protozool. 38: 489-494, 1991. PubMed: 1920148 4950: Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159 5137: Analytical microbiology. vol. 2New York: Academic Press; 1972. 21692: Alleman MM, et al. Crithidia luciliae: Effect of purine starvation on S-adenosyl-L-methionine uptake and protein methylation. Exp. Parasitol. 81: 519-528, 1995. PubMed: 8542993 23607: Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441 23820: Emtage MA, Bremner TA. Thermal regualtion of active oxygen-scavenging enzymes in Crithidia Luciliae thermophila. J. Parasitol. 79: 809-814, 1993. 23824: Goncanlves De Lima VM, et al. Comparison of six isoenzymes from 10 species of Crithidia. J. Protozool. 29: 397-401, 1982. 23870: Simione FP Jr., et al. The use of plastic ampoules for freeze preservation of microorganisms. Cryobiology 14: 500-502, 1977. PubMed: 891238 23875: Daggett PM, Decker JE. Some physiological alterations accompanying infectivity to mammals by four genera of Trypanosomatidae. Comp. Biochem. Physiol. 59: 363-366, 1978. 23887: Branquinha MH, et al. Ubiquity of cysteine-and metalloproteinase activites in a wide range of trypanosomatids. J. Eukaryot. Microbiol. 43: 131-135, 1996. PubMed: 8720943 24043: Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 24077: Freymuller E, Camargo EP. Ultrastructural differences between species of trypanosomatids with and without endosymbionts. J. Protozool. 28: 175-182, 1981. PubMed: 7024533 24177: Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays. Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515 24182: Sontheimer RD, Gilliam JN. An immunofluorescence assay for double-stranded DNA antibodies using the Crithidia luciliae kinetoplast as a double-stranded DNA substrate. J. Lab. Clin. Med. 91: 550-558, 1978. PubMed: 347011 34222: Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198 58608: Aarden LA, et al. Immunology of DNA. III. Crithidia luciliae, a simple substrate for the determination of anti-dsDNA with the immunofluorescence technique. Ann. N.Y. Acad. Sci. 254: 505-515, 1975. PubMed: 52321