Escherichia coli (Migula) Castellani and Chalmers 拉丁名
(ATCC BAA-525) 統(tǒng)一編號(hào)
Classification Enterobacteriaceae, Escherichia
Strain Designations 別名 ET12567
Application 用途 It is the key host strain for streptomyces workers worldwide.
This is a host for isolation of non-methylated DNA for transfer to other species.
This strain is used as a host to isolate DNA and plasmids for transformation into streptomyces or any other organism that has a methylation-sensitive restriction system.
Isolation 分離基物 Derived from existing strain
Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Preceptrol? no
Genotype F- dam-13::Tn9 dcm-6 hsdM hsdR zjj-202::Tn10 recF143 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtl-1 glnV44 (Douglas J MacNeil, personal communication)
Type Strain 模式菌種 no
Medium 培養(yǎng)基 ATCC? Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度: 37.0°C
Atmosphere 需氧情況: Aerobic、
Genotype F- dam-13::Tn9 dcm-6 hsdM hsdR zjj-202::Tn10 recF143 galK2 galT22 ara-14 lacY1 xyl-5 leuB6 thi-1 tonA31 rpsL136 hisG4 tsx-78 mtl-1 glnV44 (Douglas J MacNeil, personal communication)
Name of Depositor 寄存者 DJ MacNeil, T MacNeil
Special Collection NSF - Bacteriology
Isolation
Derived from existing strain
References
MacNeil DJ, et al. Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector. Gene 111: 61-68, 1992. PubMed: 1547955
constructed from strain GM2929 by T. MacNeil
Douglas J MacNeil, personal communication
Douglas J MacNeil, personal communication
Douglas J MacNeil, personal communication
