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平臺(tái)編號(hào)：bio-087095
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國(guó)際編號(hào)：ATCC 50157
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拉丁屬名： Leishmania mexicana
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
MHOM/BZ/82/BEL21
Leishmania mexicana (Biagi) Garnham 拉丁名
(ATCC? 50157?) 統(tǒng)一編號(hào)
Deposited As Leishmania mexicana mexicana (Biagi) Garnham
Strain Designations 別名 MHOM/BZ/82/BEL21
Application 用途 Vector borne research
Biosafety Level 生物安全等級(jí) 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Preceptrol?no
Isolation 分離基物 Human, Cayo District, Belize, 1982
Type Strain 模式菌種 no
Comments 注釋 WHO reference strain. Promastigotes.
Medium 培養(yǎng)基 ATCC? Medium 2831: SDM-79 Medium
ATCC? Medium 431: Trypanosome medium
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度: 25°C
Culture System: Axenic
Cryopreservation
Harvest and Preservation
1，Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.
2，Adjust concentration of cells to 2 x 107/mL in fresh medium.
3，While cells are centrifuging prepare a 10% (v
) solution of sterile DMSO in fresh medium (broth). The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
4，Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.
5，Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
6，Place the ampules in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
7，Store in either the vapor or liquid phase of a nitrogen refrigerator.
8，To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes. Do not agitate the ampule. Do not leave ampule in water bath after thawed.
9，Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh flask containing 10 mL ATCC Medium 2831.
10，Incubate at 25°C with the cap screwed on tightly.
11，Maintain as described above.
Name of Depositor 寄存者 WRAIR
Chain of Custody 來(lái)源國(guó)家 ATCC <-- WRAIR <-- DA Evans
Year of Origin 收藏年份 1982
References 參考文獻(xiàn) Evans DA, et al. The isolation and isoenzyme characterization of Leishmania braziliensis subsp. from patients with cutaneous leishmaniasis acquired in Belize. Trans. R. Soc. Trop. Med. Hyg. 78: 35-42, 1984. PubMed: 6710574
(ATCC? 50157?) 統(tǒng)一編號(hào)
Deposited As Leishmania mexicana mexicana (Biagi) Garnham
Strain Designations 別名 MHOM/BZ/82/BEL21
Application 用途 Vector borne research
Biosafety Level 生物安全等級(jí) 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Preceptrol?no
Isolation 分離基物 Human, Cayo District, Belize, 1982
Type Strain 模式菌種 no
Comments 注釋 WHO reference strain. Promastigotes.
Medium 培養(yǎng)基 ATCC? Medium 2831: SDM-79 Medium
ATCC? Medium 431: Trypanosome medium
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度: 25°C
Culture System: Axenic
Cryopreservation
Harvest and Preservation
1，Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.
2，Adjust concentration of cells to 2 x 107/mL in fresh medium.
3，While cells are centrifuging prepare a 10% (v
) solution of sterile DMSO in fresh medium (broth). The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.4，Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 5% (v
) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.5，Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
6，Place the ampules in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
7，Store in either the vapor or liquid phase of a nitrogen refrigerator.
8，To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes. Do not agitate the ampule. Do not leave ampule in water bath after thawed.
9，Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh flask containing 10 mL ATCC Medium 2831.
10，Incubate at 25°C with the cap screwed on tightly.
11，Maintain as described above.
Name of Depositor 寄存者 WRAIR
Chain of Custody 來(lái)源國(guó)家 ATCC <-- WRAIR <-- DA Evans
Year of Origin 收藏年份 1982
References 參考文獻(xiàn) Evans DA, et al. The isolation and isoenzyme characterization of Leishmania braziliensis subsp. from patients with cutaneous leishmaniasis acquired in Belize. Trans. R. Soc. Trop. Med. Hyg. 78: 35-42, 1984. PubMed: 6710574
