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Product category

Bacteria

Strain designation

Ictero No.I

Type strain

No

Genome sequenced strain

Yes

Isolation source

Blood

Geographical isolation

Japan

Applications

Agricultural research

Product format

Frozen

Storage conditions

-80°C or colder

General

Preceptrol

No

Characteristics

Serotype

icterohaemorrhagiae

Comments

Serology

Cause of Weil's Disease

Handling information

Medium

ATCC Medium 1470: Modified Leptospira medium

Temperature

30°C

Atmosphere

Aerobic

Handling procedure

Aseptically draw out cell suspension from vial and inoculate a 10?12 ml tube of Medium #1470, inserting the pipette just below the surface of the semi?solid medium.  Aliquots of 0.5 ml from this tube may be used to inoculate additional tubes if needed.  An aerobic blood plate may also be streaked to test for purity.

Incubate test tubes with screw caps lightly tightened along with the blood plate at 30°C under aerobic conditions.

Initial growth may take up to four weeks.  Observe growth as a slight, tight band forming just below the surface of the medium.  The band thickens as incubation continues.  Subsequent transfers grow in 10 to 14 days.

The aerobic blood plate should show no signs of growth.

Handling notes

Additional information on this culture is available on the ATCC web site at www.atcc.org.

Quality control specifications

Verification method

Whole-genome Sequencing

History

Deposited as

Leptospira interrogans (Stimson) Wenyon

Depositors

Y Kobayashi

Chain of custody

ATCC <-- Y Kobayashi <-- S. Yamamoto <-- I. Kubo <-- K. Sagaguchi <-- R. Inada

Type of isolate

Human

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

 

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

 

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

 

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.