Designations pBTI-9
Depositors DW Morris
Biosafety Level 安全等級(jí) 1
Vector Information
Size (kb): 10.1999998092651400
Vector: pBTI-9 (cosmid)
Construction: MUA-3, YEp13, MUA-10, YRp7
Marker(s):LEU2,tetR
Construct size (kb): 10.19999980926514
Features: marker(s): tetR, LEU2
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
vector for constructing cDNA libraries
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A Saccharomyces cerevisiae/Escherichia coli cosmid shuttle vector.
Media 培養(yǎng)基 ATCC

Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 30.0°C
References 參考文獻(xiàn)
Morris DW, et al. Plasmid vectors capable of transferring large DNA fragments to yeast. DNA 1: 27-36, 1981. PubMed: 6299664
Shipped 提供形式 freeze-dried