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 Saccharomyces cerevisiae Meyen ex E.C. Hansen 拉丁名

ATCC 200808 編號

Deposited As Saccharomyces cerevisiae Hansen
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations 菌株別名 YM4271
Alternate State Candida robusta Diddens et Lodder
Application
transformation host
transformation host for selecting DNA binding sites with colony sectoring assay
Biosafety Level 安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Storage Conditions 培養(yǎng)條件

Frozen 冷凍物 : -80°C or colder
Freeze-Dried 凍干物 : 2°C to 8°C
Live Culture  : See Propagation Section
Type Strain 模式菌株 no
Genotype MATa ura3-52 his3-delta200 ade2-101 lys2-801 leu2-3 leu2-112 trp1-901 gal4-delta512 gal80-delta538 ade5::hisG
Preceptrol no
Mating Type a
Ploidy haploid
Comments
Note: This material is available under the conditions that you will not use it for commercial purposes or distributed it to third parties.
Morphology On YPAD medium at 25°C after 7 days, colonies white to buff, smooth, butyrous, margin entire becoming slightly eroded. Cells hyaline, subglobose to broadly ellipsoidal, smooth, 6-7.5 X 4.5-6 ?m. Pseudohyphae not observed.
Medium ATCC Medium 1245: YEPD
ATCC 培養(yǎng)基 Medium 1069: YPAD medium
Growth Conditions 生長條件 
Temperature 培養(yǎng)溫度 : 24°C to 26°C
Atmosphere: Typical aerobicSequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAATGGATTTTTTTGTTTTGGCAAGAGCATGAGAGCTTTTACTGGGCAAGAAGACAAGAGATGGAGAGTCCAGCCGGGCCTGCGCTTAAGTGCGCGGTCTTGCTAGGCTTGTAAGTTTCTTTCTTGCTATTCCAAACGGTGAGAGATTTCTGTGCTTTTGTTATAGGACAATTAAAACCGTTTCAATACAACACACTGTGGAGTTTTCATATCTTTGCAACTTTTTCTTTGGGCATTCGAGCAATCGGGGCCCAGAGGTAACAAACACAAACAATTTTATCTATTCATTAAATTTTTGTCAAAAACAAGAATTTTCGTAACTGGAAATTTTAAAATATTAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCCTTCTCAAACATTCTGTTTGGTAGTGAGTGATACTCTTTGGAGTTAACTTGAAATTGCTGGCCTTTTCATTGGATGTTTTTTTTCCAAAGAGAGGTTTCTCTGCGTGCTTGAGGTATAATGCAAGTACGGTCGTTTTAGGTTTTACCAACTGCGGCTAATCTTTTTTTATACTGAGCGTATTGGAACGTTATCGATAAGAAGAGAGCGTCTAGGCGAACAATGTTCTTAAAGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAA
D1D2 region of the 28S ribosomal RNA gene
ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGTGTTTTGTGCCCTCTGCTCCTTGTGGGTAGGGGAATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGCCTCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCGC
Morphology On YPAD medium at 25°C after 7 days, colonies white to buff, smooth, butyrous, margin entire becoming slightly eroded. Cells hyaline, subglobose to broadly ellipsoidal, smooth, 6-7.5 X 4.5-6 ?m. Pseudohyphae not observed.
Name of Depositor M Johnston
Special Collection NCRR Contract
Chain of Custody 來源國家 ATCC <-- M Johnston <-- J.D. Liu
References 參考文獻(xiàn)
Liu J, et al. Identifying DNA-binding sites and analyzing DNA-binding domains using a yeast selection system. Methods 5: 125-137, 1993.
Luo Y, et al. Cloning and analysis of DNA-binding proteins by yeast one-hybrid and one-two-hybrid systems. BioTechniques 20: 564-568, 1996. PubMed: 8800670