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平臺(tái)編號(hào)：bio-104444
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國際編號(hào)：ATCC 76273
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拉丁屬名： Komagataella phaffii
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規(guī)格：freeze-dried
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
NRRL Y-11430
Komagataella phaffii Kurtzman 拉丁名
ATCC 76273 編號(hào)
Deposited As Komagataella pastoris
Strain Designations 菌種別名 NRRL Y-11430 [21-1, CBS 7435]
Application 用途 Produces alcohol oxidase.
Produces single-cell protein SCP.
Biosafety Level 安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 培養(yǎng)條件
Frozen 冷凍物 : -80°C or colder
Freeze-Dried 凍干物 : 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌株 no
Preceptrol no
Genome Sequenced Strain
Yes
Comments 注釋
Genome sequencing strain (The Institute of Molecular Biotechnology, Graz University of Technology, Austria).
Mitochondrial genome sequenced (The Institute of Molecular Biotechnology, Graz University of Technology, Austria).
Morphology On YM medium at 25°C after 8 days, colonies creamy white, butyrous, dull, smooth, margin entire. Cells hyaline, globose to subglobose, smooth, 3-7 X 3-6 ?m. Pseudohyphae not observed.
Medium 培養(yǎng)基ATCC Medium 28: Emmons' modification of Sabouraud's agar
ATCC Medium 200: YM agar or YM broth
ATCC Medium 1245: YEPD
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 24°C to 26°C
Atmosphere 需氧情況 : Typical aerobic
Sequenced Data
Internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence
TAAATCTCCTCCCAACGGATCTCTTGGCTCTCGCGACGATGAAGAACGCAGCGATCCGCGATACGTCCTGGAAGCCGCCGTGAACCATCAATTTTCGAACGCACATTGCATACAGGGAGGGTGGGGTGAGTCTACTCTCACTATCGTGGTTTAAATACAAAGGATGACTAATACAATCGTCAGCCCCCAAATTTTCTCCCCCGCCCCCTCAGATTACCCGCTGAACTTAAGCATATCAATAA
D1D2 region of the 26S ribosomal RNA gene
ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAAAGTCCACCTTTGAAGTATTGACGTGGTTGGGAGAGGCAATGTGCACAAGTCGCCTGGAACGGCCGCCAAAGAGGGTGATAGCCCCGTAGCACGGTCAACCTTCCCAGCCGACAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAAGGTGGTAGGGTCCATCAAAGACTAAATATCAGCGAGAGACCGATAGCAAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAACAGTGCGTGAAATTGGGGGGAGGGAAGGCAAGGCCACGACAAGTGGTCCTGCGC
Morphology On YM medium at 25°C after 8 days, colonies creamy white, butyrous, dull, smooth, margin entire. Cells hyaline, globose to subglobose, smooth, 3-7 X 3-6 ?m. Pseudohyphae not observed.
Name of Depositor 寄存者 NRRL
Chain of Custody 來源國家 ATCC <-- NRRL <-- Phillips Petroleum Co. 21-1
References 參考文獻(xiàn)
Wegner EH. Biochemical conversions by yeast fermentation at high cell densities. US Patent 4,617,274 dated Oct 14 1986
Hopkins TR. Alcohol oxidase from Pichia-type yeasts. US Patent 4,619,898 dated Oct 28 1986
Kuberl A, et al. High-quality genome sequence of Pichia pastoris CBS7435. J. Biotechnol. 20;154(4): 312-320,2011. PubMed: 21575661
Kurtzman CP. Biotechnological strains of Komagataella (Pichia) pastoris are Komagataella phaffii as determined from multigene sequence analysis. J Ind Microbiol Biotechnol 36: 1435-1438, 2009. PubMed: 19760441
