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Cryptococcus gattii (Vanbreuseghem et Takashio) Kwon-Chung et Boekhout 拉丁名
(ATCC? MYA-4877?) 統(tǒng)一編號(hào)
Strain Designations 別名 A6MR38 [CBS 11545]
Alternate State Filobasidiella bacillispora Kwon-Chung, teleomorph
Biosafety Level 生物安全等級(jí) 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Type Strain no
Genotype VGIIc; AFLP6C
Preceptrol? no
Mating Type MATalpha
Comments 注釋 highly virulent novel genotype Ref
Morphology On YM medium at 25°C after 6 days, colonies creamy white to pale yellow, mucoid. Cells globose to subglobose, smooth, contain oil droplets, 3-5.25 ?m X 3-5.25 ?m. Pseudohyphae not observed.
Medium 培養(yǎng)基 ATCC? Medium 200: YM agar or YM broth
ATCC? Medium 1245: YEPD
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度: 20°C to 25°C
Atmosphere 需氧情況: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.
GTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATACTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATG
TGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACAT
CGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAA
GGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCAGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTC
GGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACA
ACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAA


D1D2 region of the 28S ribosomal RNA gene:
ACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGA
AACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGA
TACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAAC
AAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTA
TTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGG
GGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTG
ACAAAATGGCTTTAAACGACCCGTCTT

Morphology On YM medium at 25°C after 6 days, colonies creamy white to pale yellow, mucoid. Cells globose to subglobose, smooth, contain oil droplets, 3-5.25 ?m X 3-5.25 ?m. Pseudohyphae not observed.
Name of Depositor CBS
Special Collection ATCC
Chain of Custody ATCC <-- CBS <-- K Bartlett, E Burne, F Hagen
Isolation
CSF of immunicompetent human, Oregon, USA
Cross References
Nucleotide (GenBank) : JQ812708 ITS including 5.8S rRNA gene
Nucleotide (GenBank) : JQ812702 D1/D2 region of LSU rRNA gene
References
MacDougall, L., et al. Spread of Cryptoccus gattii in British Columbia, Canada, and detection in the Pacific Northwest, USA. Emerg. Infect. Dis. 13(1): 42-50, 2007. PubMed: 17370514
Byrnes EJ III, et al. Emergence and pathogenicity of highly virulent Cryptococcus gattii genotypes in the Northwest United States. PLoS Pathog. 6: e1000850, 2010. PubMed: 20421942
Byrnes EJ III, et al. Molecular evidence that the Vancouver Island Cryptococcus gattii outbreak has expanded into the United States Pacific Northwest. J. Infect. Dis. 199: 1081-1086, 2009. PubMed: 19220140
Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334