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平臺編號：bio-109102
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國際編號：ATCC 3002
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拉丁屬名： Trypanosoma brucei
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
Trypanosoma brucei
Trypanosoma brucei gambiense Dutton 拉丁名
ATCC 30025 編號
Deposited As Trypanosoma gambiense Dutton
Strain Designations Wellcome Ts
Application 用途
Vector borne research
Biosafety Level 安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation Human, Hospital for Tropical Diseases, London, England, 1921
Product Format 提供形式 frozen
Storage Conditions Frozen Cultures:
-70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures:
2-8°C
Live Cultures:
See Protocols section for handling information
Type Strain 模式菌株 no
Growth Conditions 生長條件
Culture System 需氧情況 : in vivo, laboratory rat
Cryopreservation Reagents
Tyrode's Salt Solution
NaCl, 8.00 g
KCl, 0.20 g
CaCl2, 0.20 g
MgCl2 ? H2O, 0.05 g
NaH2PO4 ? H2O 1.00 g
NaHCO3 ? H2O, 1.00 g
Glucose, 1.00 g
Glass distilled H2O to 1.00 L
Add ingredients in the sequence listed. Filter-sterilize.
Harvest and Preservation
Harvest the parasites according to the protocol for maintenance in vivo.
Spin the cell suspension at approximately 50 x g for 3 min, to remove any cellular debris.
Transfer the supernatant to a new 15 mL plastic centrifuge tube. Centrifuge at 1300 x g for 10 min.
Pool the cell pellets and adjust the concentration to 2.0 - 4.0 x 107 cells/mL with a fresh solution of Tyrode's Salt Solution. *If the concentration is too low centrifuge at 1300 x g for 10 min and resuspend in the volume of Tyrode's Salt Solution required to yield the desired concentration.
Mix the cell preparation and 10% (v) DMSO in equal portions. The final concentration will be 1.0 - 2.0 x 107 cells/mL and 5% DMSO. The time from the mixing of the cell preparation and cryoprotective solution to the start of the freezing process should be no less than 15 min. and no more than 30 min.
Dispense in 0.5 mL aliquots to 1.0-2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
Store in either the vapor or liquid phase of a nitrogen refrigerator.
To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes. Do not agitate the ampule. Do not leave ampule in water bath after thawed.
Immediately after thawing, aseptically remove the contents of the ampule with a syringe and inoculate an uninfected rat. Follow the protocol for maintenance in vivo.
Name of Depositor 寄存者 EJ Tobie
Chain of Custody 來源國家 ATCC <-- EJ Tobie <-- C.A. Hoare
Year of Origin 1921
References 參考文獻
. . J. Parasitol. 36: 48-54, 1950.
Southworth GG, Read CP. Carbohydrate transport in Trypanosoma gambiense. J. Protozool. 16: 720-723, 1969. PubMed: 5362387
