Escherichia coli GFP 拉丁名
(ATCC® 25922GFP™) 統(tǒng)一編號
Verified ByWhole-genome Sequencing
ApplicationFluorescence detection
Fluorescence imaging
FACS
Cell infection assays
In vivo localization
Pathogen-host interactions assays
Biosafety Level生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Antigenic Properties血清型 Serotype 06, Biotype 1
Product Format提供形式 frozen
Storage Conditions 保藏條件 Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol®no
Type Strain 模式菌種 no
Antibiotic ResistanceAmpicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 100 µg/mL ampicillin.
Comments 注釋 This clone was derived from ATCC® 25922™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Plac promoter. This construct has been designed for Gram-negative bacteria fluorescence labeling.
Vector: pUCP18-MCSgfpmut3
Vector size: 5.492kb
Vector type: plasmid
Marker: bla (ampR)
Features:
GFPmut3: fluorescent maker under the control of Plac promoter
Origin of replication: ori from pRO1600
Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI.
Double restriction sites: HindIII, PstI, SalI, XbaI.
MediumATCC® Medium 2855: Tryptic Soy Agar/Broth with 100 mcg/ml Ampicillin
Growth Conditions 培養(yǎng)溫度 Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor 寄存者 M Barbier
Chain of CustodyModified by ATCC (Dr. M. Barbier) <-- ATCC <-- FDA <-- F Schoenknecht
References 參考文獻(xiàn)
Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster.
Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227
