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Human respiratory syncytial virus  拉丁名

ATCC VR-1580 編號(hào)

Classification Paramyxoviridae, Pneumovirus 

Deposited As Respiratory syncytial virus 

Agent Human respiratory syncytial virus 

Strain 18537 

Verified By Whole-genome Sequencing 

Applications 用途 Respiratory research 

Positive for Mycoplasma No 

Biosafety Level 生物安全等級(jí) 2 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 1 mL per vial 

Storage Conditions 保藏條件 -70°C or colder 

Comments The early passage history of this item is unknown. 

Effect on Host CPE, massive syncytia formation and sloughing 

Recommended Host HEp-2 (ATCC? CCL-23?) 

Growth Conditions 生長(zhǎng)條件 

Temperature 培養(yǎng)溫度 : 37°C 

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. 

Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). 

Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3-7 daysEffect on Host CPE, massive syncytia formation and sloughing 

Name of Depositor RM Chanock, J Crowe, National Institute of Allergy and Infectious Diseases; ATCC 

Special Collection ATCC 

Source Respiratory secretions from child with acute respiratory disease seen at Children’s Hospital of the District of Columbia, Washington, DC, 1962. 

Treated ATCC? VR-1401? to remove mycoplasma at ATCC?. 

References 參考文獻(xiàn) Coates HV, et al. An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am. J. Epidemiol. 83: 299-313, 1966. PubMed: 5933417