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 Betacoronavirus 1
VR-1558?
Product category   Viruses
Product type   Animal virus
Classification   Coronaviridae, Betacoronavirus
Strain designation   OC43
Common name   Human coronavirus OC43
Type strain   No
Isolation source   Man with cold-like illness. Treated to remove mycoplasma at ATCC.
Applications   Infectious disease research
Respiratory disease research
Product format    Frozen
Storage conditions    -70°C or colder
Specific applications
Respiratory research
Characteristics
Comments
VR-1558? is a TC-adapted item derived from virus passaged in mice (ATCC? VR-759?).  Cleaned of mycoplasma with MRA.  No antigenic cross reaction with Human coronavirus 229E (ATCC? VR-740?).

Next-generation sequencing (NGS) at ATCC on the HCT-8 [HRT-18] cell line (ATCC? CCL-244?) used as the host has shown the presence of a putative fragment of Gammaherpesvirus with a ≥98% homology to Human Heparanase 2 (NCBI number NG_023416.1).
Mycoplasma contamination
Not detected
Handling information
Host
HCT-8 [HRT-18] (ATCC CCL-244)
Effects
CPE; cell vacuolization; cell sloughing
Complete medium
RPMI-1640 (ATCC? 30-2001?) + 2% HS (ATCC? 30-2040?)
Temperature
33°C
Incubation
4-6 days at 33°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2atmosphere, while rocking continuously. End adsorption by adding virus growth medium.
Handling notes
VR-1558? is a TC-adapted item derived from virus passaged in mice (ATCC? VR-759?).  Cleaned of mycoplasma with MRA.  No antigenic cross reaction with Human coronavirus 229E (ATCC? VR-740?).
Key abbreviations
RPMI, Roswell Park Memorial Institute
TC, Tissue culture
CPE, Cytopathic effect
HCT-8, adult human colon epithelial cells
HS, Horse serum
MOI, Multiplicity of infection
MRA, Mycoplasma removal agent
PBS, Phosphate-buffered saline
Quality control specifications
Volume
1.0 mL
Verification method
Whole-genome Sequencing
History
Deposited as
Human coronavirus OC43
Depositors
JO Fleming, University of Wisconsin, Madison
Chain of custody
ATCC <-- JO Fleming <-- MK Cooney <-- AZ Kapikian
Type of isolate
Human
Patient gender
Male
Cross references
GenBank NC_005147.1 Human coronavirus OC43, complete genome