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平臺編號：bio-117524
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國際編號：ATCC 27665
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拉丁屬名： Spiroplasma citri
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規(guī)格： Freeze-dried
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
Spiroplasma citri
Spiroplasma citri Saglio et al.拉丁名
27665? 統(tǒng)一編號
Product category:Bacteria
Strain designation:[California (C-189), NCPPB 2648]
Type strain模式菌種 :No
Isolation隔離源：source:Stubborn disease-infected navel orange seedling
Product format提供形式:Freeze-dried
Preceptrol:No
Characteristics
Antigenic properties Group I, subgroup 1
Handling information
Medium培養(yǎng)基:ATCC Medium 668: SMC medium
Temperature培養(yǎng)溫度：32°C
Atmosphere需氧條件：Anaerobic
Handling procedure
1. Open vial according to enclosed instructions.
2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and plate under anaerobic conditions at 37°C. Incubate blood plate aerobically at 37°C.
5. Within 24 to 48 hours, growth should be evident by turbidity in the broth. No growth should occur on the blood agar plate incubated aerobically.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
1. Open vial according to enclosed instructions.
2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and plate under anaerobic conditions at 32°C. Incubate blood plate aerobically at 32°C.
5. Within 24 to 48 hours, growth should be evident by turbidity in the broth. No growth should occur on the blood agar plate incubated aerobically.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Handling notes
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
Additional information on this culture is available on the ATCC web site at www.atcc.org
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
Additional information on this culture is available on the ATCC web site at www.atcc.org
History
Deposited as
Spiroplasma citri Saglio et al.
Depositors
JG Tully
Chain of custody
ATCC <-- JG Tully <-- J.M. Bove strain California (C-189)
Type of isolate
Plant
27665? 統(tǒng)一編號
Product category:Bacteria
Strain designation:[California (C-189), NCPPB 2648]
Type strain模式菌種 :No
Isolation隔離源：source:Stubborn disease-infected navel orange seedling
Product format提供形式:Freeze-dried
Preceptrol:No
Characteristics
Antigenic properties Group I, subgroup 1
Handling information
Medium培養(yǎng)基:ATCC Medium 668: SMC medium
Temperature培養(yǎng)溫度：32°C
Atmosphere需氧條件：Anaerobic
Handling procedure
1. Open vial according to enclosed instructions.
2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and plate under anaerobic conditions at 37°C. Incubate blood plate aerobically at 37°C.
5. Within 24 to 48 hours, growth should be evident by turbidity in the broth. No growth should occur on the blood agar plate incubated aerobically.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
1. Open vial according to enclosed instructions.
2. Under anaerobic conditions, withdraw 0.5 ml of recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube. A slant and a pre-reduced blood plate may also be inoculated with 0.1 ml each of the cell suspension. An aerobic blood plate may also be streaked to check for purity.
4. Incubate tubes and plate under anaerobic conditions at 32°C. Incubate blood plate aerobically at 32°C.
5. Within 24 to 48 hours, growth should be evident by turbidity in the broth. No growth should occur on the blood agar plate incubated aerobically.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
Handling notes
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
Additional information on this culture is available on the ATCC web site at www.atcc.org
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
Additional information on this culture is available on the ATCC web site at www.atcc.org
History
Deposited as
Spiroplasma citri Saglio et al.
Depositors
JG Tully
Chain of custody
ATCC <-- JG Tully <-- J.M. Bove strain California (C-189)
Type of isolate
Plant
