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新型隱球菌Cryptococcus neoformans var. grubii Franzot et al. 拉丁名
ATCC 204092 編號(hào)
An ampoule containing viable cells suspended in cryoprotectant.
Product category Fungi
Product type Yeast
Strain designation 別名 Vitek 302898 [9.887]
Type strain 模式菌株 No
Product format 提供形式 Frozen
Storage conditions 保藏溫度 -80°C or colder
Specific applications Biomedical Research and Development Material Emerging infectious disease research
Preceptrol No
Comments This yeast was re-identified as Cryptococcus neoformans var. grubii based on the multigene DNA sequence comparison.
Medium 培養(yǎng)基 ATCC Medium 28: Emmons' modification of Sabouraud's agar
ATCC Medium 200: YM agar or YM broth
ATCC Medium 1245: YEPD
Temperature 培養(yǎng)溫度 30°C
Atmosphere 需氧情況 Aerobic
Handling procedure Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.
If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.
Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C).
Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material.
Do not agitate the ampoule. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 μL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Deposited as Cryptococcus neoformans (Sanfelice) Vuillemin
Depositors bioMerieux Vitek, Inc.
Chain of custody 來源國(guó)家 ATCC <-- bioMerieux Vitek, Inc. <-- Medical College of Virginia 9.887
Special collection NCRR Contract
Intended use This product is intended for laboratory research use only.
It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.