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Filobasidiella neoformans Kwon-Chung, teleomorph 拉丁名

ATCC 34873 編號

 An ampoule containing viable cells (e.g. yeast cells, spores) suspended in cryoprotectant. 

Product category Fungi 

Product type Yeast 

Strain designation 別名 NIH B-3501 [CBS 6900, CBS 7697, CBS 7817, CCRC 20532] 

Type strain 模式菌株 No 

Genome sequenced strain Yes 

Isolation 分離源 source Single-basidiospore isolate from a cross of ATCC 28957 x ATCC 28958. 

Product format 提供形式 Freeze-dried 

Storage conditions 保藏條件 2°C to 8°C 

Specific applications Biomedical Research and Development Material assay of eumelanin pigments Ref Ref produces monophenol monooxygenase phenol oxidase, phenoloxidase Ref regulation of phenol oxidase and capsular polysaccharide Ref Preceptrol NoSerotype D Mating type alpha Genotype VNIV Morphology After 3 days on YM medium at 25°C, colony is cream-colored, mucoid. Cells are globose, single. No hyphae observed. 

Comments Designated as type strain of Cryptococcus deneoformans Hagen et Boekhout by Hagen et al. (2015). Genome sequencing strain (The University of Oklahoma, USA). Smooth colony type Ref

Medium 培養(yǎng)基 ATCC Medium 28: Emmons' modification of Sabouraud's agar 

ATCC Medium 200: YM agar or YM broth 

ATCC Medium 1245: YEPD 

Temperature 培養(yǎng)溫度 25-30°C 

Atmosphere 需氧情況 Aerobic Handling procedure For freeze-dry (lyophilized) ampoules: Open an ampoule according to enclosed instructions. 

From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension. 

Aseptically transfer the suspension back into the test tube of sterile distilled water. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi. Mix the suspension well. 

Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum. Incubate the inoculum at the propagation conditions recommended. Inspect for growth of the inoculum/strain regularly. 

The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain. 

Sequenced data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTTGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGC

ACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCATATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAA

TGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAAT

CTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCT

GTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGA

ACTTAAGCATATCAATAA D1D2 region of the 26S ribosomal RNA gene

 ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTG

TAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATA

CGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGA

AAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTAT

TCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTG

AGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC 26S ribosomal RNA gene and intergenic spacer (IGS), partial sequence

 ATGGGAACGGGGTGCTGTAAGTGGTAGAGTAGCCTTGTTGCTACGATCCACTGAGGCTAAGCCCTTGTTCTATAGATTTGTCTCTAACATGTTGGGTCTCGGGGGGCTTCC

TCTAGAGACTTGGATGTAAGGGGCTTTACGCATTCACATAATATTAGTTGGGGACTGAGGGGGGTGTGTGCTGTGCGAGCCCGGCTGATAATTGTTTTAATTGTTAGCTTGG

ATTGCACACACAGTCTCATCAGTCTTCAGCTTGGGTTATCGATATTCTGTAAGTCCAAACTTATCCATTCACATTCACTGTAGTGTAGGTTGCAGAGAGGACTTGGAAAGTTCT

CTCTCTGCACTGGGCATACTACTGATTTTATGGTCATGGGGGACTTGGGAGCTGGTGCTTGTGTCGCATAAGGTAGAGTTGATGGTTGGACAGATAGATAAGCATTTTTATGT

CGCGGGGGACTTGGGGTAAGACGCCTTGCAGCAAGTAAGAGAGTCAAACAGTAGTTACTTGTTCTAGTCGTGGGGGACTTTGGTAGTGGTAGTGAGCCGGAATGAGAGATGG

ATAAATGCAGTATGGATGGTGACAGGAGGAGCAGCAGAGCGTATGCAGTGGATATGTATATAGTTGAGAAGGGATGGACAGTAGAACGTTTGAACGTTGAGGAATAATTGACC

CAAGGGTGATTCTAATAGACTTGTCAAGAAAAAGTTAAGTTGAATCAAGCTTGGTCAAGCAAAGTCTAGAAAAGATTTACAATCACCAAGAATTGCCCGAGTCGCGGGGGGCTT

GTCACTTCTCTTGCTCATTGTGGGTCCAGTCT glycerol-3-phosphate dehydrogenase 1 (GPD1) gene, partial cds

 CAACGGTTTCGGTACGTAATCAATCCTTCTCTTCCCGTTGCTTCATATCCCACCGATGTGACTTCCAATATTGTGCGCTGGCTGTTCTTGAGTTATGGTAGCTAATAGCTATGTGA

TAGGTCGTATTGGTCGAATTGTTCTCAGGTATGCTTTCTTTAAGTCAACGGGGTAAGAAGCCCGTGTATCTAACGTTCAGGTAGGAACGCCATCGAGCATGGGGACCTCGAGGTT

GTTGCTGTCAACGAGTGAGTGTCCCAAAAGTGTTAATCCATTAGATGAATCACTTAATTGTCCATAGCCCTTTTATTGACTTAGACTACATGGTATGATACTTCTTTCCGCCCAATA

GCTTCGTACTGACTCTGTTGCGCAGGTCTACATGTTCAAGTATGACTCCGTAAGTCTCCCAAGTTCTAACGTTCTGTATGGCTTATTAACCTGATTTTCGACAGACACATGGTCGCT

TCAAGGGCTCCGTCGAGGTTAAGGACGGTAAACTTTACATCAACAACAAGGCCATTGCCGTCTTCGGCGAGAAAGACCCTGCCAACATCAAGTGGGG 

History 來源歷史 Deposited as Cryptococcus neoformans (Sanfelice) Vuillemin Depositors KJ Kwon-Chung 

Cross references 查詢文獻(xiàn) GenBank AF316889 Filobasidiella neoformans var. neoformans elongation factor 3 mRNA, complete cds.Intended use This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.