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Bacillus subtilis subsp. subtilis (Ehrenberg) Cohn 拉丁名

ATCC 35021 編號(hào)

Product category Bacteria 

Strain designation 5230 [NRS 6] 

Type strain No 

Applications Bioinformatics 

Storage conditions 保藏溫度 -80°C or colder 

Specific applications Sterilization control 

Preceptrol No 

Comments Heat resistance

Medium 培養(yǎng)基 ATCC Medium 18: Trypticase Soy Agar/Broth 

Temperature 培養(yǎng)溫度 30°C 

Atmosphere 需氧情況 Aerobic 

Handling procedure Open vial according to enclosed instructions. Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. 

Rehydrate the entire pellet. 

Aseptically transfer this aliquot back into the broth tube. Mix well. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate. Incubate all tubes and plate at 30°C for 24 hours. 

Verification method Whole-genome Sequencing

Deposited as Bacillus subtilis (Ehrenberg) Cohn Depositors Travenol Laboratories 

Chain of custody 來源國家 ATCC <-- Travenol Laboratories <-- C.R. Schmidt 5230 <-- H.C. Curran 15mu