Providencia stuartii (Buttiaux et al.) Ewing
25825
Product category
Bacteria
Strain designation
NCDC 247
Type strain
No
Isolation source
Pericardium at post mortem
Geographical isolation
Italy
Product format
Freeze-dried
Storage conditions
2°C to 8°C BSL 1
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen. General
Preceptrol
No
Characteristics
Serotype
O20:H4
Handling information
Medium
ATCC Medium 3: Nutrient agar or nutrient broth
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
Open vial according to enclosed instructions.
Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the broth tube. Mix well.
Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
Incubate all tubes and plate at 37°C for 24 hours.
Handling notes
On #3 plates, colonies are entire, glistening, smooth, translucent, and gray
