Mycoplasma salivarium 拉丁名
ATCC 23064 編號(hào)
Type strain 模式菌株
Product category
Bacteria
Strain designation 別名 [H110, NCTC 10113, PG 20]
Type strain 模式菌株 Yes
Isolation 分離源 source Saliva
Product format 提供形式 Freeze-dried
Storage conditions 保藏方法 2°C to 8°C
Medium 培養(yǎng)基 ATCC Medium 243: Mycoplasma medium
Temperature 溫度 37°C
Atmosphere 需氧情況 Anaerobic
Handling procedure
Follow instructions as suggested for the culturing of Mollicutes:
Open the vial according to the enclosed instructions.
Using a Pasteur or 1.0 ml pipette, withdraw approximately 0.5 to 1.0 ml from a tube containing 5.0 ml. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the tube. Mix well.
Make serial dilutions by transferring 0.2 ml from the original tube to a tube containing 2.25 ml. Repeat process by transferring 0.25 ml from the second to a third tube, etc. Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth. Many strains will die out rapidly once acid or alkaline conditions are reached. It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze drying process often inhibits growth.
Use an uninoculated tube of broth to serve as a control.
Plates may be inoculated to check colonial morphology. You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony forming units. However, not all strains do well on solid medium.
Incubate all tubes and plates under the recommended conditions and appropriate temperature. The time necessary for growth will vary from strain to strain. Growth on plates generally requires additional incubation.
Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both.
Turbidity appears in the first few dilution tubes within 3-5 days. Additional incubation is required for colonies to appear on solid medium.
Subsequent fresh transfers will grow between 24-48 hours. This strain produces good turbidity. Colonies on plates are visible to the naked eye on prolonged incubation.
ATCC23064
