Feline infectious peritonitis virus
VR-1777™
Product category
Viruses
Classification
Coronaviridae, Alphacoronavirus, Alphacoronavirus 1
Strain designation
FIPV-1146
Isolation source
Isolate from neonatal kitten, Washington State University, 1979. Treated to remove Mycoplasma hyorhinis at ATCC.
Geographical isolation
United States; Washington; Pullman
Product format
Frozen
Storage conditions
-70°C or colder
BSL 2
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Characteristics
Comments
Three freeze-thaw cycles performed at harvest. Zoopathogenic. Plaque purified FIPV 79-1146 virus (approximately passage 11), was used to develop attenuated Feline infectious peritonitis virus strains. Cross-reacts with Canine coronavirus, Transmissible gastroenteritis virus of swine, and Human coronavirus 229E.
Handling information
Host
A-72 (ATCC CRL-1542)
Effects
cell rounding; cell sloughing; CPE
Complete medium
DMEM (ATCC® 30-2002™) + 2% FBS (ATCC® 30-2020™)
Temperature
34°C
Atmosphere
95% Air, 5% CO2
Incubation
2-4 days
Recommendation for infection
Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 34°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Handling notes
Three freeze-thaw cycles performed at harvest. Cross-reacts with canine coronavirus, TGE virus of swine, and Human coronavirus 229E.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
CPE, Cytopathic effect
FIPV, Feline infectious peritonitis virus
MOI, Multiplicity of infection
TGE, transmissible gastroenteritis
Quality control specifications
Volume
1.0 mL
History
Depositors
F Scott, Cornell University
Chain of custody
ATCC <-- F Scott
Year of origin
1979
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
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