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美國(guó)ATCC>
ATCC菌種類>
艱難梭菌Clostridium difficile (Hall and O'Toole) Prevot 拉丁名
(ATCC BAA-1803) 統(tǒng)一編號(hào)
Application 用途 Enteric Research
Isolation 分離基物 Clinical specimen
Biosafety Level 生物安全等級(jí) 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Preceptrol?no
Genotype Toxinotype: IIIc;
Type Strain 模式菌種 no
Comments 注釋 Presence of tcdA and tcdB genes confirmed by PCR.
Ref
Confirmed by CDC: NAP1, Toxinotype III, binary toxin positive
Binary Toxin gene cdtB was amplified by PCR
Toxinotype IIIc
Ribotype 027
Medium 培養(yǎng)基 ATCC? Medium 2107: Modified Reinforced Clostridial
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度: 37°C
Atmosphere 需氧情況: Anaerobic
Genotype Toxinotype: IIIc;
Name of Depositor 寄存者 R Clark
Isolation 分離基物 Clinical specimen
References 參考文獻(xiàn) Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Stubbs, S et al. Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile. FEMS Microbiol. Lett. 186(2): 307-312, 2000. PubMed: 10802189
Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986