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Clostridium perfringens (Veillon and Zuber) Hauduroy et al.  拉丁名

ATCC 19574 編號(hào)

Toxinotype A  毒型A

Product category Bacteria Product type Anaerobe 

Strain designation 別名 43F4 

Type strain 模式菌株 No 

Isolation 分離源 source Bile Geographical isolation United States; Maryland; Baltimore 

Applications Agricultural research Enteric disease research Infectious disease research 

Product format 提供形式 Freeze-dried 

Storage conditions 保藏條件 2°C to 8°C

Specific applications Enteric Research 

Preceptrol No

Genotype cpa, cpe, cpb2, pfoA Toxigenic Yes Toxin genes cpa (Alpha toxin) positive cpb2 (Beta2 toxin) positive cpe (Enterotoxin) positive etx (Epsilon toxin) negative pfoA (Perfringolysin O) positive 

Comments Toxinotype A 

Hydrolyzes bile acid conjugates The presence of cpa, cpe, cpb2, and pfoA genes was confirmed by PCR. 

This strain does not produce epsilon toxin

Medium 培養(yǎng)基 ATCC Medium 2107: Modified Reinforced Clostridial 

ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood 

Temperature 培養(yǎng)溫度 37°C 

Atmosphere 需氧情況 Anaerobic 

Handling procedure Open vial according to enclosed instructions or visit www.atcc.org for instructions. 

Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #2107 broth. 

Aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth. 

Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. 

Best practice dictates the use of pre-reduced media. Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant. 

Incubate in an anaerobic atmosphere at 37°C for 24 hours. Incubate one agar plate aerobically at 37°C to check for contamination. 

Deposited as Clostridium perfringens (Veillon and Zuber) Hauduroy et al. Depositors JF Reback Type of isolate Human