Streptococcus pneumoniae (Klein) Chester
10354 ™
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Bacterial strain with applications in respiratory disease research. This strain is serotype 54 (Danish designation 15B).
Product category
Bacteria
Type strain
No
Genome sequenced strain
Yes
Applications
Infectious disease research
Respiratory disease research
Vaccine development
Product format
Freeze-dried
Storage conditions
2°C to 8°C
General
Specific applications
Respiratory research
Preceptrol
No
Characteristics
Serotype
54 (Danish designation 15B)
Handling information
Medium
ATCC Medium 44: Brain Heart Infusion Agar/Broth
ATCC Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Handling procedure
Open vial.
Rehydrate the entire pellet with approximately 0.5 mL of #44 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #44 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
Incubate at 37°C for 24 hours in an atmosphere of 5% CO2.
Handling notes
Resuspending preserved culture in Trypticase Soy broth with 5% defibrinated sheep blood can be done as an alternative for growth. Additional transfers may be made into BHI broth. Shaking may improve growth. Additional transfers may be made into BHI broth. Shaking may improve growth.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
Quality control specifications
Verification method
Whole-genome Sequencing
History
Deposited as
Diplococcus pneumoniae (Klein) Weichselbaum
Depositors
BE Eddy
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