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Quantitative Synthetic Monkeypox virus DNA 拉丁名

ATCC VR-3270SD 編號

ATCC Quantitative Synthetic Monkeypox virus DNA can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. 

The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. Preparation includes fragments from J2L, D14L, F3L, F8L, A27L, A29L, B6R, B7R, and N3R regions. 

The sequence of this research use only (RUO) construct was designed based on several published assays. 

Product category Viruses 

Product type Molecular standard Nucleic acid 

Organism Monkeypox virus, MPXV 

Applications 用途 Infectious disease research Assay development Zoonotic disease research 

Specification range 規(guī)格范圍 ≥ 1 x 105 to 1 x 106 copies/μL 

Volume 規(guī)格 100 μL 

Product format 提供形式 Frozen 

Shipping information Shipped in a proprietary stabilization matrix 

Storage conditions 保藏溫度 -20°C or colder

Specific applications ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.

Genetic target Preparation includes fragments from J2L, D14L, F3L, F8L, A27L, A29L, B6R, B7R, and N3R regions. 

Comments 注釋 The sequence of this research use only (RUO) construct was designed based on several published assays. 

Manufactured under ISO 13485 guidance 

The recipient testing laboratory is responsible for generating validation or verification data as applicable to establish performance characteristics as required by the testing laboratory’s policies, applicable regulations, and quality system standards.

Handling procedure Thaw the vial on ice. Avoid exposing the synthetic DNA to repeated freeze-thaw cycles as it may result in degradation of the DNA and variation in copy number. 

Gently mix the sample to ensure an even distribution of material. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom. 

Handling notes Aliquoting is highly recommended to avoid multiple freeze-thaws, which can damage the synthetic DNA. 

This construct is synthetically derived and therefore does not contain any viable material and cannot replicate. 

The following primers and probe can be used with this nucleic acid preparation. Forward primer (5’ to 3’): CATCTATTATAGCATCAGCATCAGA 

Reverse primer (5’ to 3’): GATACTCCTCCTCGTTGGTCTAC 

Probe (5’ to 3’): JOE/TGTAGGCCGTGTATCAGCATCCATT/BHQ1

Quality accreditation Manufactured under ISO 13485 guidance

Depositors ATCC