Myceliophthorathermophila(Apinis)vanOorschot
48104?
Anampoulecontainingviablecells(e.g.yeastcells,spores)suspendedincryoprotectant.
Productcategory
Fungi
Straindesignation
D-14
Typestrain
No
Isolationsource
Garbage
Geographicalisolation
Italy
Productformat
Freeze-dried
Storageconditions
2°Cto8°C
General
Specificapplications
Producesamylase
Producesendoglucanase
Producesglucosidase,beta;acidGlucosidase,beta,acid,glucocerebrosidase
Producesthermostableamylase
Degradescellulosicwastes
Preceptrol
No
Characteristics
Morphology
After10daysonPotatodextrosemediumat45°C,coloniesarebuffturninglightbrownwithage,velutinous;reversebrown.Hyphaehyalinetoyellow,guttulate.Conidiaovoid,hyaline,smooth-walled,bornesinglyorinmassesfromshortprotrusions.
Handlinginformation
Medium
ATCCMedium325:Maltextractagar(Blakeslee'sformula)
ATCCMedium336:Potatodextroseagar(PDA)
Temperature
37-50°C
Atmosphere
Aerobic
Handlingprocedure
Forfreeze-dry(lyophilized)ampoules:
Openanampouleaccordingtoenclosedinstructions.
Fromasingletesttubeofsteriledistilledwater(5to6mL),withdrawapproximately0.5to1.0mLwithasterilepipetteandapplydirectlytothepellet.Stirtoformasuspension.
Asepticallytransferthesuspensionbackintothetesttubeofsteriledistilledwater.
Letthetesttubesitatroomtemperaturefor(25°C)undisturbedforatleast2hours;longer(e.g.,overnight)rehydrationmightincreaseviabilityofsomefungi.
Mixthesuspensionwell.Useseveraldrops(ormakedilutionsifdesired)toinoculaterecommendedsolidorliquidmedium.
Incubatetheinoculum/strainatthetemperatureandconditionsrecommended.
Inspectforgrowthoftheinoculum/strainregularly.Thesignofviabilityisnoticeabletypicallyafter3-5daysofincubation.However,thetimenecessaryforsignificantgrowthwillvaryfromstraintostrain.
Handlingnotes
producesamylase,producesendoglucanase,producesglucosidasebetaacid,producesglucocerebrosidase,producesthermostablyamylase,degradescellulosicwaste.
Additional,updatedinformationonthisproductmaybeavailableontheATCCwebsiteatwww.atcc.org
Qualitycontrolspecifications
Sequenceddata
18SribosomalRNAgene,partialsequence;internaltranscribedspacer1,5.8SribosomalRNAgene,andinternaltranscribedspacer2,completesequence;and28SribosomalRNAgene,partialsequence.
CCAGCGGAGGGATCATTACAGAGTTGCAAAACTCCCTAAACCATCGTGAACGCTACCTAAACCGTTGCTTCGGCGGGCGGCGCCCTCGCGCGCCCCCCTGGGGCCCGCACCGCGGGCGCCCGCCGGAGGTACACCAAACTCTTGATATGTTATGGCCACTCTGAGTCTCCTGTACTGAATAAGTCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGCATCCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCATCAAGCCCACGGCTTGTGTTGGGGACCTGCGGCTGCCCGCAGGCCCTGAAAACCAGTGGCGGGCTCGCTAGTCACACCGGGCGTAGTAGCATACGACCTCGCTCAGGGCGTGCTGCGGGTTCCAGCCGTAAAACGACCTTCAGAACCCAAGGTTGACCTCGGATCAGGTAGGAGGACCCGCTGAACTTAA
D1D2regionofthe28SribosomalRNAgene
CATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTTCGGCCCGAGTTGTAATTTGCAGAGGAAGCTTTAGGCGCGGCACCTTCTGAGTCCCCTGGAACGGGGCGCCATAGAGGGTGAGAGCCCCGTATAGTTGGATGCCTAGCCTGTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGGGAGGTAAATTTCTTCTAAAGCTAAATACCGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAATAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGTGACCAGACTTGCGCCGGGCTGATCATCCGGTGTTCTCACCGGTGCACTCTGCCCGGCTCAGGCCAGCATCGGTTCTCGCGGGGGGATAAAGGCCCGGGGAATGTAGCTCCTCCGGGAGTGTTATAGCCCCGGGTGTAATACCCTCGCGGGGACCGAGGTTCGCGCATCTGCAAGGATGCTGGCGTAATGGTCATCAGCGAC
History
Depositedas
Myceliophthorathermophila(Apinis)vanOorschot
Depositors
SLChakrabarty
Legaldisclaimers
Intendeduse
Thisproductisintendedforlaboratoryresearchuseonly.Itisnotintendedforanyanimalorhumantherapeuticuse,anyhumanoranimalconsumption,oranydiagnosticuse.
Warranty
Theproductisprovided'ASIS'andtheviabilityofATCC?productsiswarrantedfor30daysfromthedateofshipment,providedthatthecustomerhasstoredandhandledtheproductaccordingtotheinformationincludedontheproductinformationsheet,website,andCertificateofAnalysis.Forlivingcultures,ATCCliststhemediaformulationandreagentsthathavebeenfoundtobeeffectivefortheproduct.Whileotherunspecifiedmediaandreagentsmayalsoproducesatisfactoryresults,achangeintheATCCand/ordepositor-recommendedprotocolsmayaffecttherecovery,growth,and/orfunctionoftheproduct.Ifanalternativemediumformulationorreagentisused,theATCCwarrantyforviabilityisnolongervalid.Exceptasexpresslysetforthherein,nootherwarrantiesofanykindareprovided,expressorimplied,including,butnotlimitedto,anyimpliedwarrantiesofmerchantability,fitnessforaparticularpurpose,manufactureaccordingtocGMPstandards,typicality,safety,accuracy,and/ornoninfringement.
Disclaimers
Thisproductisintendedforlaboratoryresearchuseonly.Itisnotintendedforanyanimalorhumantherapeuticuse,anyhumanoranimalconsumption,oranydiagnosticuse.AnyproposedcommercialuseisprohibitedwithoutalicensefromATCC.
WhileATCCusesreasonableeffortstoincludeaccurateandup-to-dateinformationonthisproductsheet,ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureandpatentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeenconfirmedtobeaccurateorcompleteandthecustomerbearsthesoleresponsibilityofconfirmingtheaccuracyandcompletenessofanysuchinformation.
Thisproductissentontheconditionthatthecustomerisresponsibleforandassumesallriskandresponsibilityinconnectionwiththereceipt,handling,storage,disposal,anduseoftheATCCproductincludingwithoutlimitationtakingallappropriatesafetyandhandlingprecautionstominimizehealthorenvironmentalrisk.Asaconditionofreceivingthematerial,thecustomeragreesthatanyactivityundertakenwiththeATCCproductandanyprogenyormodificationswillbeconductedincompliancewithallapplicablelaws,regulations,andguidelines.Thisproductisprovided'ASIS'withnorepresentationsorwarrantieswhatsoeverexceptasexpresslysetforthhereinandinnoeventshallATCC,itsparents,subsidiaries,directors,officers,agents,employees,assigns,successors,andaffiliatesbeliableforindirect,special,incidental,orconsequentialdamagesofanykindinconnectionwithorarisingoutofthecustomer'suseoftheproduct.Whilereasonableeffortismadetoensureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfromthemisidentificationormisrepresentationofsuchmaterials.