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Epizoot--ic hemorrh--agic disease virus 2
VR-1897™
Product categoryViruses
Product typeAnimal virus
ClassificationSedoreovirinae, Orbivirus, Epizootic hemorrhagic disease virusStrain designationOV215Isolation sourceSpleen of a farmed white-tailed deer, Gadsden County, Florida. Isolated on October 11, 2016.Geographical isolationUnited States; FloridaApplicationsInfectious disease research
Vector-borne disease research

Product formatFrozenStorage conditions-70°C or colder

HostVERO C1008 [Vero 76, clone E6, Vero E6] (ATCC CRL-1586)
EffectsCPE; cell rounding; cell sloughing
Complete mediumEMEM (ATCC® 30-2003™) + 2% FBS (ATCC® 30-2020™)
Temperature37°C
Atmosphere95% Air, 5% CO2
Incubation2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.Recommendation for infectionPlate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.Handling notesStrain fully sequenced by depositorKey abbreviations°C, Degrees Celsius
CO2, Carbon dioxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection