Mycoplasma hyorhinis 拉丁名
ATCC 23234 編號
Product category Bacteria
Strain designation 別名 [PG42; BTS-7]
Type strain 模式菌株 No
Product format 提供形式 Freeze-dried
Preceptrol No
Comments Same as ATCC 17981, q.V
Medium 培養(yǎng)基 ATCC Medium 243: Mycoplasma medium
Temperature 培養(yǎng)溫度 37°C
Atmosphere 需氧情況 95% Air, 5% CO2
Handling procedure PROPAGATION PROCEDURE:
1. Follow instructions as suggested for the culturing of Mollicutes: PROCEDURES FOR PROPAGATING MOLLICUTES:
a) Open the vial according to the enclosed instructions.
b) Using a Pasteur or 1.0 ml pipette, withdraw approximately 0.5 to 1.0 ml from a tube containing 5.0 ml. Rehydrate the pellet.
c) Aseptically transfer this aliquot back into the tube. Mix well.
d) Make serial dilutions by transferring 0.5 ml from the original tube to a tube containing 4.5 ml. Repeat process by transferring 0.5 ml from the second to a third tube, etc. Dilutions are important, not only for titration purposes, but also to keep culture in varying stages of growth. Many strains will die out rapidly once acid or alkaline conditions are reached. It is recommended to prepare several dilutions from the initial tube as the cryoprotectant used in the freeze?drying process often inhibits growth.
e) Use an uninoculated tube of broth to serve as a control.
f) Plates may be inoculated to check colonial morphology. You can also spot each dilution on the surface of plate (4 or more/plate) to determine the number of colony-forming units. However, not all strains do well on solid medium.
g) Incubate all tubes and plates under the recommended conditions and appropriate temperature. The time necessary for growth will vary from strain to strain. Growth on plates generally requires additional incubation.
h) Depending on the medium used, growth will be indicated by increased turbidity, a color change, or both.
2. Tubes may be incubated aerobically, but plates are incubated under 5% CO2. The incubation temperature is 37oC.
3. This strain will show turbidity in the first few dilution tubes within four to five days. Additional incubation will be required for colonies to appear on solid medium.
4. Subsequent, fresh transfers will grow up in three to four days. This strain produces very light turbidity. You may have to hold it up to a light source to see.
Deposited as Mycoplasma hyorhinis Switzer Depositors RG Wittler
Chain of custody ATCC <-- RG Wittler <-- D.G. ff. Edward PG 42 <-- W.P. Switzer BTS-7
