亚日韩在线中文字幕亚洲,国产在线播放鲁啊鲁视频,张柏芝手扒性器全部图片,亚洲成无码电影在线观看

Product category
Viruses
Product type
Animal virus
Classification
Picornaviridae, Enterovirus, Human enterovirus A
Strain designation
Gdula
Genome sequenced strain
Yes
Isolation source
Stool from a child with sore throat, fever and leg muscle spasm.
Geographical isolation
United States; New York
Product format
Frozen
Storage conditions
-70°C or colder
Derived by adaptation of primary cell culture product ATCC VR-165 to RD cells (ATCC CCL-136).  ATCC VR-165 and ATCC VR-1801 have not been compared for sequence of infectivity in primary cell culture and tissue culture.
Handling information
Host
RD (ATCC CCL-136)
Effects
CPE; cell death; cell rounding
Complete medium
DMEM (ATCC 30-2002) + 10% FBS (ATCC 30-2020)
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Incubation
2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Recommendation for infection
For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer with DPBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Handling notes
Derived by adaptation of primary cell culture product ATCC VR-165 to RD cells (ATCC CCL-136).  ATCC VR-165 and ATCC VR-1801 have not been compared for sequence of infectivity in primary cell culture and tissue culture.
Key abbreviations
CPE, Cytopathic effect
DMEM, Dulbecco's Modified Eagle's Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
D-PBS, Dulbecco's Phosphate-buffered saline
RD, Rhabdomyosarcoma cells
Quality control specifications
Volume
1.0 mL
Verification method
Whole-genome Sequencing
History
Depositors
HA Wenner
Type of isolate
Human
Year of origin
1949
Patient age
5 years
Legal disclaimers
Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty
The product is provided 'AS IS' and the viability of ATCC? products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.