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Feline infectious peritonitis virus 拉丁名

VR-1812 編號

Product category Viruses 

Product type Animal virus 

Classification Coronaviridae, Alphacoronavirus, Alphacoronavirus 1 Strain designation FIPV-1146, P80 Genome sequenced strain Yes 

Isolation 分離源 source FIPV-WSU-1146 isolated from necropsy of a feline neonate with pneumonia and pleuritis. 

Product format 提供形式  Frozen 

Storage conditions -70°C or colder 

Comments Live attenuated virus derived from serial passage of plaque-purified FIPV 79-1146, a strain isolated by J. Evermann. Intranasal administration of passage 80 protects adult cats from challenge by virulent virus. Cross reacts with canine coronavirus, TGE virus of swine and human coronavirus 229E. Host A-72 (ATCC CRL-1542) Effects cell rounding; cell sloughing; CPE Complete medium Leibovitz's L-15 (ATCC® 30-2008™) + 10% FBS (ATCC® 30-2020™) 

Temperature 培養(yǎng)溫度 34°C 

Atmosphere 需氧情況 95% Air, 5% CO2 

Incubation 1-3 days at 34°C in air atmosphere, humidified, until CPE is progressed through 80% of the monolayer. 

Recommendation for infection For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. 

Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). 

Wash monolayer one time with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 34°C in air atmosphere, humidified, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. 

Handling notes Live attenuated virus derived from serial passage of plaque-purified FIPV 79-1146, a strain isolated by J. Evermann. Intranasal administration of passage 80 protects adult cats from challenge by virulent virus. 

Cross reacts with canine coronavirus, TGE virus of swine and human coronavirus 229E. This item has been treated with Mycoplasma Removal Agent (MRA) to remove Mycoplasma at the ATCC. The previous item number was ATCC® VR-2201™. Key abbreviations A-72, Canine fibroblast tumor cells CPE, Cytopathic effect FBS, Fetal bovine serum L-15, Leibovitz's L-15 Medium MOI, Multiplicity of infection PBS, Phosphate-buffered saline TGE, transmissible gastroenteritisVolume 1.0 mL Verification method Whole-genome SequencingDepositors FW Scott Type of isolate Animal